FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3072586
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC150110.48854613019782034No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT97710.3180057449978617No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT63440.2064710312420873No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC60110.19563325485438002No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA46340.15081758492683361No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT45770.14896247004965849No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA45640.14853937367416242No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA43820.14261602441721727No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT43080.14020762966439343No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT42350.13783178078660777No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG41720.13578139065920367No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA40760.13265698665554032No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA37910.1233814122696647No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT37390.12168902676768038No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA36520.11885753563936047No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC33330.1084754015021874No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA30880.1005016621178382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA57700.0123.261174
AGCACGC9850.0119.973251
GCACGCA10200.0115.856542
CACGCAC10400.0113.628523
GAAACAC21800.0109.41394
TAACAAG66000.0107.8699955
AAACACG22250.0106.87525
CGGTGCA4250.0104.057652
GCCGACC1300.0100.38511145
TAGAAAC27650.092.2955252
TAGTAAC80900.088.630032
ACGTGCA10500.085.618082
ACGCACT14450.084.28974
CGCACTT14750.082.575335
AACACGT6200.081.853916
AGTAACA89150.080.753433
TTCGGAA755.638867E-1177.33372145
AACACGA26850.070.474066
ACAAGAG101600.070.144387
GAAGCGG6850.069.853221
Produced by FastQC (version 0.11.5)