FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751309
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAAAGTTCC48560.17649780522653036No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC45200.16428543649586433No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT45040.1637038951277374No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA44890.16315870009511838No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA42500.154471925908722No Hit
GGATAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA38070.13837049927870698No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA37950.13793434325261178No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT36530.13277316361048505No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA34790.12644890123210442No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT34100.12394100408205694No Hit
CTATTATCACATCCTTTTGATTTAATATATGTTATCTAGGTTTAAAACAA33850.12303234569435859No Hit
CTATTATCCAGGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT32340.11754404903266044No Hit
GCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGG31500.11449095684999396No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA29770.10820304080712126No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT29030.10551341197953411No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG28980.10533168030199443No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA28550.10376878787515326No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT27870.10129723706061369No Hit
GTAGTAACAAGAGCAGAATTTTAAAACAAAAAACTTATAAAAAGGAAATA27820.10111550538307401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA13100.096.2966462
AGCACGC14050.091.849521
TAGTAAC80150.080.775782
GTAACAA80300.079.722034
GTAGTAA82250.079.0661
GCGGTGC9600.075.521
AGTAACA90250.071.816383
AACAACG14450.063.2172977
ACAACGC14700.062.635368
TAACAAG103850.062.0623865
GAAGCGG11950.058.2420351
CGACGAA6850.058.211033145
CACGCAC22150.057.6065863
AAGAGCG21150.057.5880179
CAACGCA15950.057.27219
CGACGCC400.00605227254.3744145
GCCAGCG19500.053.5378727
CGCACTT23000.053.271155
ACGCACT26700.051.8627244
AACAAGA124800.051.4697886
Produced by FastQC (version 0.11.5)