FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008805
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT158580.5270530991539831No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC119110.39587145062574675No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT98030.32581041310420583No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA52570.17472052858194534No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC51490.17113106366148687No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG48420.1609276772672207No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT46290.15384845478520542No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA45240.15035869722364859No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA38880.12922073713650437No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA37760.12549832907084374No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT36530.12141032735587716No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT35310.11735556142721114No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA34970.11622554469299273No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA32860.10921279378357854No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT32010.10638775194803253No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA31680.10529097100011467No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG31570.1049253773508087No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG30810.10239945759196757No Hit
GTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTCTTAGTGGAG30140.10017265990983132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC25000.0128.759281
GCACGCA25200.0127.737382
CACGCAC25450.0127.052333
GAAACAC16000.0116.9055944
CGGTGCA5450.0114.403032
AAACACG16950.0110.353375
CGCACTT33250.099.646065
ACGCACT33550.098.538944
GTAACAA69450.091.2377854
GCGGTGC12050.090.8501051
AACACGT5000.088.44956
TAACAAG74250.084.949025
ACGTGCA9700.082.9634552
ACAACGC8200.082.225158
GTCGCCC950.076.31536145
CGGTGCG4500.074.1106952
CAACGCA9150.073.689339
GGTGCGT4450.073.314193
GACAGGT502.3074463E-672.4995961
TGACGCC2100.072.49959145
Produced by FastQC (version 0.11.5)