FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904967
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTAGACAGTGCCGTTCAATGTTTTCAAATCTTGTTACTATTACTCTT185890.9758174288583477No Hit
CTCATATACCTTCCAATTGATGTTTTTTTGAAACACCCTTAGACAGTGCC165560.8690964200429718No Hit
GTCTAAGGGTGTTTCAAAAAAACATCAATTGGAAGGTATATGAGAGAAAA155820.8179669254113062No Hit
GTAGTAACAAGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGA149780.7862603394179533No Hit
GTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACA131140.6884108753589957No Hit
GTAGAAACACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGA110190.5784352169880108No Hit
GTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGT108560.5698786383176191No Hit
CTTTAATATATTGGGGTTTCATGAGAGTCTTAGTGTCATAAAAAGTTCTG88250.4632626181975855No Hit
GTCTTAGTGTCATAAAAAGTTCTGCTGCTAAAAGAGTAATAGTAACAAGA81450.4275664617812277No Hit
GAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCG79010.41475784094947576No Hit
ATATTAAAGAGGTAAAATCAGGGATAGAGGATTCATAGCCATACCTGATC74380.39045295797774976No Hit
ACTTAGAGTCACCACTTCATTTCTCTGGGCTCTAGTTAATTCATGTATAA68810.361213606324939No Hit
GGCAAAGGCTGCAACGAGGCCGACGACGCATTCACGCTGGCGCGCCGGCG67810.3559641715578275No Hit
TCGTAGAACACCACGCGCTTGCTGGCGCCGAAGCCGGTGAAGTACGCATT67780.3558066885148142No Hit
GAAGCGGAGCGGGCACTTGAAGCAAATTTTACGGGGGGTGTTCATATGGT61770.32425758556447437No Hit
GTAGAAACACGTGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGA61510.32289273252502537No Hit
GCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTG60570.3179582638439406No Hit
GCTAAAAGAGTAATAGTAACAAGATTTGAAAACATTGAACGGCACTGTCT56680.297537962599877No Hit
GGCCCAGCTCGTGCGCCAGCACGGCCTCGACCTCGCCGGCGCTGAGCTGC55850.29318093174317456No Hit
GCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGT53530.281002243083476No Hit
GTTCTACGACACGCTGCTGCGGCAGCTCAGCGCCGGCGAGGTCGAGGCCG53490.28079226569279153No Hit
GCTTGAAATGGCCCAGCTCGTGCGCCAGCACGGCCTCGACCTCGCCGGCG52440.2752803591873245No Hit
GCCTGTAACAATATAATAAAAGAGGCAGACATGTTGAAGACAGATTTTCC52200.2740204948432177No Hit
GGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGCCTCGTTG51800.27192072093637315No Hit
ATCCTTAACAAAGCCAAGAAAACCCACAGCCTGTAACAATATAATAAAAG51360.2696109696388441No Hit
GTTAGAATTGTGGTTTTCAAACTGCTCTTCAGAGCTCTGAGAAGGTAGTT50250.26378409704735045No Hit
TGCCAATGCGTACTTCACCGGCTTCGGCGCCAGCAAGCGCGTGGTGTTCT50070.26283919878927037No Hit
ATATTGGGGTTTCATGAGAGTCTTAGTGTCATAAAAAGTTCTGCTGCTAA49560.26016198705804355No Hit
GTATAAAGCCATAGTGGTCTGTTAGAATTGTGGTTTTCAAACTGCTCTTC49270.2586396509755812No Hit
CTATTACCGCTTCTCGTTGCGCTTGGATTCACTGTTGCAGAAGTAGAATC48910.2567498544594211No Hit
CAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGACCAT48530.25475506924791874No Hit
ATTTCAAGCACCGCCACATCGTCAAGCGCATGGTCACGCTGTTCGCGCTG47880.2513429366492963No Hit
AGTAGAAACAACGCACTTGGCCAAGCTTGTCTGTCTGTTCGTTCTTTCTT47220.24787830970300273No Hit
AGCTGGGCCATTTCAAGCACCGCCACATCGTCAAGCGCATGGTCACGCTG46870.24604100753451374No Hit
GTATATGAGAGAAAAGAAGACCTTGAAAATGGGGAAAATCTGTCTTCAAC46760.24546356971013147No Hit
AAACACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCT46530.24425619971369583No Hit
CTGCTGCGGCAGCTCAGCGCCGGCGAGGTCGAGGCCGTGCTGGCGCACGA45980.24136901059178453No Hit
GCGCTTGACGATGTGGCGGTGCTTGAAATGGCCCAGCTCGTGCGCCAGCA45930.24110653885342895No Hit
AGTTAATTCATGTATAAAGCCATAGTGGTCTGTTAGAATTGTGGTTTTCA42980.22562070629045017No Hit
GCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCA41970.2203187771756676No Hit
GTAGAACACCACGCGCTTGCTGGCGCCGAAGCCGGTGAAGTACGCATTGG41920.22005630543731206No Hit
GCGTGAATGCGTCGTCGGCCTCGTTGCAGCCTTTGCCAGCGCACATGCCA41610.2184289806595075No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA41140.21596174631896511No Hit
GGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGCCT40930.21485936501787173No Hit
GTTGAAGACAGATTTTCCCCATTTTCAAGGTCTTCTTTTCTCTCATATAC40210.21107977198555145No Hit
CTTCACCGGCTTCGGCGCCAGCAAGCGCGTGGTGTTCTACGACACGCTGC39860.20924246981706246No Hit
GTGTTCTACGACACGCTGCTGCGGCAGCTCAGCGCCGGCGAGGTCGAGGC39610.2079301111252846No Hit
GTAGTAACAAGAGCAAGAACTATTACCGCTTCTCGTTGCGCTTGGATTCA39520.20745766199624455No Hit
GAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCG39450.20709020156254673No Hit
GACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGT39050.20499042765570216No Hit
GTTGCAGCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGCTTCGG38980.20462296722200435No Hit
AAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGC38540.20231321592447532No Hit
CACATACCCTTTTCCAACTACCTTCTCAGAGCTCTGAAGAGCAGTTTGAA38190.20047591375598633No Hit
TTTTCAAGGTCTTCTTTTCTCTCATATACCTTCCAATTGATGTTTTTTTG38090.1999509702792752No Hit
AGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGCC37950.19921604941187956No Hit
GCAGAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGC37780.19832364550147064No Hit
GAGCAGAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCG37760.1982186568061284No Hit
GCCTCGTTGCAGCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGC37410.1963813546376394No Hit
GAGTAATAGTAACAAGATTTGAAAACATTGAACGGCACTGTCTAAGGGTG36950.19396661464476814No Hit
GAATTAACTAGAGCCCAGAGAAATGAAGTGGTGACTCTAAGTTGCTTGAA36910.19375663725408365No Hit
AAACAACAGCATCCTTAACAAAGCCAAGAAAACCCACAGCCTGTAACAAT36480.19149938030422575No Hit
GCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGCTTCGGCGCCAG35750.1876672929242344No Hit
GGCATGTGCGCTGGCAAAGGCTGCAACGAGGCCGACGACGCATTCACGCT35500.1863549342324565No Hit
GCCTCGACCTCGCCGGCGCTGAGCTGCCGCAGCAGCGTGTCGTAGAACAC35330.18546253032204757No Hit
CACTAAGACTCTCATGAAACCCCAATATATTAAAGAGGTAAAATCAGGGA33910.17800833295274932No Hit
TCTCAGAGCTCTGAAGAGCAGTTTGAAAACCACAATTCTAACAGACCACT33770.1772734120853537No Hit
TGCTGGCGCCGAAGCCGGTGAAGTACGCATTGGCATGTGCGCTGGCAAAG33640.17659098556562924No Hit
GATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGCCTCGTTGCAG32940.17291638122865122No Hit
GGTCGAGGCCGTGCTGGCGCACGAGCTGGGCCATTTCAAGCACCGCCACA32810.17223395470892672No Hit
CTATTACTCTTTTAGCAGCAGAACTTTTTATGACACTAAGACTCTCATGA32520.1707116186264644No Hit
CGTTGCAGCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGCTTCG32470.17044914688810883No Hit
ATGTGGCGGTGCTTGAAATGGCCCAGCTCGTGCGCCAGCACGGCCTCGAC32130.16866433906729092No Hit
TAACAAGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCT32070.16834937298126426No Hit
GCGTACTTCACCGGCTTCGGCGCCAGCAAGCGCGTGGTGTTCTACGACAC31840.1671420029848286No Hit
CAGAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCG31800.16693202559414416No Hit
CCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGACC31730.16656456516044635No Hit
GAAGTACGCATTGGCATGTGCGCTGGCAAAGGCTGCAACGAGGCCGACGA31200.16378236473387728No Hit
GGCCAAGCTTGTCTGTCTGTTCGTTCTTTCTTTCTTTCTTTCTTTCTTTC30550.16037023213525484No Hit
GCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGACCATGCGCTTGACG30100.15800798649005468No Hit
CGCCAGCACGGCCTCGACCTCGCCGGCGCTGAGCTGCCGCAGCAGCGTGT29960.15727306562265908No Hit
CTACGACACGCTGCTGCGGCAGCTCAGCGCCGGCGAGGTCGAGGCCGTGC29460.15464834823910337No Hit
GCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGACCATGC29440.15454335954376114No Hit
TCACCGGCTTCGGCGCCAGCAAGCGCGTGGTGTTCTACGACACGCTGCTG29360.1541234047623922No Hit
CACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAG29100.15275855172294323No Hit
GTCATAAAAAGTTCTGCTGCTAAAAGAGTAATAGTAACAAGATTTGAAAA28240.1482440378232274No Hit
GCCATACCTGATCTCACATACCCTTTTCCAACTACCTTCTCAGAGCTCTG27930.14661671304542284No Hit
CCTTCCAATTGATGTTTTTTTGAAACACCCTTAGACAGTGCCGTTCAATG27910.1465117243500806No Hit
TTAATATATTGGGGTTTCATGAGAGTCTTAGTGTCATAAAAAGTTCTGCT27890.14640673565473838No Hit
GGCTGCAACGAGGCCGACGACGCATTCACGCTGGCGCGCCGGCGCTTCCA27260.14309959175145817No Hit
AACTATTACCGCTTCTCGTTGCGCTTGGATTCACTGTTGCAGAAGTAGAA26920.14131478393064026No Hit
GAAGCAGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCG26770.14052736871557356No Hit
ACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGAC26670.14000242523886242No Hit
TAATAAAAGAGGCAGACATGTTGAAGACAGATTTTCCCCATTTTCAAGGT26540.13931999871913792No Hit
GAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGG26190.1374826965506489No Hit
GTGTTCATATGGTCAATTTTGGGGGAAGGCATAGGCTAAATTTTTTCTTC26020.13659029264023997No Hit
ATTAGTAGAAACACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCC25860.13575038307750215No Hit
TCTCTGGGCTCTAGTTAATTCATGTATAAAGCCATAGTGGTCTGTTAGAA25260.13260072221723526No Hit
AACAAGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTC25210.13233825047887968No Hit
AGCAGAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGG24890.13065843135340402No Hit
GGGATAGAGGATTCATAGCCATACCTGATCTCACATACCCTTTTCCAACT24840.13039595961504846No Hit
CTCATATACCTTCCAATTGATGTTTTTTTGAAACACCCTTAGACCTGTCT24510.12866364614190168No Hit
CGCTTGCTGGCGCCGAAGCCGGTGAAGTACGCATTGGCATGTGCGCTGGC24510.12866364614190168No Hit
ACCATGCGCTTGACGATGTGGCGGTGCTTGAAATGGCCCAGCTCGTGCGC24380.12798121962217718No Hit
AGTAGAAACAACGCACTCGGCCAAGCTTGTCTGTCTGTTCGTTCTTTCTT24300.12756126484080826No Hit
AGCGTGAATGCGTCGTCGGCCTCGTTGCAGCCTTTGCCAGCGCACATGCC24140.12672135527807044No Hit
CACGTGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAG24060.12630140049670152No Hit
AAGCAGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGC23870.12530400789095034No Hit
AAGCCATAGTGGTCTGTTAGAATTGTGGTTTTCAAACTGCTCTTCAGAGC23670.12425412093752805No Hit
TTGCAGCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGCTTCGGC23620.1239916491991725No Hit
GCCATAGTGGTCTGTTAGAATTGTGGTTTTCAAACTGCTCTTCAGAGCTC23490.123309222679448No Hit
AACACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTC23140.12147192051095898No Hit
GTCTAAGGGTGTTTCAAAAAAACATCAATTGGAAGGTATATGAGCTGTCT22990.12068450529589227No Hit
GACATGTTGAAGACAGATTTTCCCCATTTTCAAGGTCTTCTTTTCTCTCA22910.12026455051452334No Hit
ACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTG22730.11931965225644328No Hit
GCTCTTCAGAGCTCTGAGAAGGTAGTTGGAAAAGGGTATGTGAGATCAGG22200.11653745182987421No Hit
GTGCTGGCGCACGAGCTGGGCCATTTCAAGCACCGCCACATCGTCAAGCG22140.11622248574384754No Hit
TCATGAGAGTCTTAGTGTCATAAAAAGTTCTGCTGCTAAAAGAGTAATAG21980.11538257618110971No Hit
GTAACAAGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGC21980.11538257618110971No Hit
GAACACCACGCGCTTGCTGGCGCCGAAGCCGGTGAAGTACGCATTGGCAT21600.11338779096960734No Hit
AACTAGAGCCCAGAGAAATGAAGTGGTGACTCTAAGTTGCTTGAATGCTA21520.11296783618823844No Hit
GCGTGACCATGCGCTTGACGATGTGGCGGTGCTTGAAATGGCCCAGCTCG21350.11207543227782948No Hit
CTTCAGAGCTCTGAGAAGGTAGTTGGAAAAGGGTATGTGAGATCAGGTAT21030.11039561315235383No Hit
CAGCGTGACCATGCGCTTGACGATGTGGCGGTGCTTGAAATGGCCCAGCT20980.11013314141399824No Hit
CCCTTTTCCAACTACCTTCTCAGAGCTCTGAAGAGCAGTTTGAAAACCAC20350.10682599751071803No Hit
GTCAAGCGCATGGTCACGCTGTTCGCGCTGAGCCTCGCGGGCTTCGCGCT20310.10661602012003357No Hit
CACTTGGCCAAGCTTGTCTGTCTGTTCGTTCTTTCTTTCTTTCTTTCTTT20180.10593359360030907No Hit
CAGCGCGAACAGCGTGACCATGCGCTTGACGATGTGGCGGTGCTTGAAAT20090.10546114447126904No Hit
GAGCTGCCGCAGCAGCGTGTCGTAGAACACCACGCGCTTGCTGGCGCCGA20040.10519867273291349No Hit
GGCATAGGCTAAATTTTTTCTTCGACAAGCGGGGCCGATTCTACTTCTGC20030.10514617838524237No Hit
GGTATGGCTATGAATCCTCTATCCCTGATTTTACCTCTTTAATATATTGG19790.10388631404113563No Hit
CTGTTAGAATTGTGGTTTTCAAACTGCTCTTCAGAGCTCTGAGAAGGTAG19740.10362384230278004No Hit
ATTTTGGGGGAAGGCATAGGCTAAATTTTTTCTTCGACAAGCGGGGCCGA19640.1030988988260689No Hit
GTAACAAGATTTGAAAACATTGAACGGCACTGTCTAAGGGTGTTTCAAAA19510.10241647230634442No Hit
TTCATAGCCATACCTGATCTCACATACCCTTTTCCAACTACCTTCTCAGA19390.10178654013429105No Hit
CATAAAAAGTTCTGCTGCTAAAAGAGTAATAGTAACAAGATTTGAAAACA19170.10063166448552653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA36750.0102.189131
GAGCAGA18950.081.107121
AAGAGCG35950.073.406549
CGAGCGA406.6651715E-572.49904145
GGAGCGG15800.071.1224756
CGGAGCG16150.070.927865
TGCCGCC6200.066.65234145
GAGTCGA350.00356718662.142033145
AGCGGGC22750.061.823368
ACAACGC19850.061.359398
AACAACG20400.060.0604867
CAGAGCG4750.059.525535
CGGTGCA5750.059.260092
CAACGCA20700.059.1900379
GCGAGGG753.7633617E-757.999233145
GCTAACC502.0108398E-457.99923145
AAGCGGA48850.057.2868542
TCTCGGG8750.056.342113145
TAGTAGT8550.055.9641723
CAAGAGC66400.055.9028748
Produced by FastQC (version 0.11.5)