FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017771
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC110150.3650045016669588No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT109150.3616907976118798No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT96480.31970616723402806No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA56580.18748937543637342No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC50540.16747460294369587No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG47970.15895838352214267No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT43630.14457690792309955No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA36250.12012177199661606No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC35280.11690747906318935No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA31900.10570715935702212No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG31080.10298992203185729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA24200.0120.132592
AGCACGC24550.0118.12461
CACGCAC26150.0110.897093
CGCACTT29600.0100.910745
ACGCACT31050.097.365774
CGGTGCA8750.096.112922
AAACACG23250.094.1707155
GAAACAC23700.092.076764
TAACAAG85850.091.542095
GTAACAA96200.081.768594
ACGTGCA15350.080.764332
GTCTCGC8150.080.060221
TAGTAAC101500.077.6417542
GCGGTGC17250.076.911961
GTAGTAA105900.075.511211
AGTAACA111100.071.3896263
CTCGCTC8800.070.027423
AACACGT9150.069.7257846
CGCTCTG8850.068.8125845
ACAAGAG121950.064.621887
Produced by FastQC (version 0.11.5)