FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831612
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC83260.4545722565696228No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT72150.3939153052065612No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC51990.28384832595549714No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT51650.2819920376149534No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT47040.2568229515858162No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT32350.176620375931147No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA31710.17312618611365288No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT31520.17208884851158435No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA26960.14719274606193888No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG26690.14571863473268357No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA25730.1404773500064424No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC25070.13687396675715163No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT23580.12873905608829816No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT23570.12868445937239983No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA21360.11661858515886551No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA21170.11558124755679695No Hit
CAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTT20920.11421632965933834No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC20150.11001238253516574No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT19790.10804690076282532No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC19490.10640899928587495No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA18890.10313319633197425No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT18880.10307859961607589No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC18770.1024780357411941No Hit
TAATATATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACC18670.10193206858221063No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT18660.1018774718663123No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA100.007091528144.998722
GTAACAA43150.0129.205114
AGCACGC5150.0122.474651
GCACGCA5300.0117.6404652
CGGTGCA3650.0117.190752
TAACAAG47850.0116.665645
CACGCAC5300.0116.272553
GAAACAC13250.0108.8858264
AAACACG13150.0108.6112065
TAGTAAC56600.099.0141452
ACGCACT6950.093.884064
TTCGGAA850.093.8227145
CGCACTT6900.092.462955
AGTAACA61250.091.378783
TAGAAAC17300.088.424072
CTTCACG259.3883334E-486.999232
ACAAGAG67550.082.641757
AACACGT4150.075.119826
GGTGCGT2650.073.867273
CACGGTC300.00193606872.499365
Produced by FastQC (version 0.11.5)