FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992834
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT78290.2615915216146301No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG64020.21391096198452703No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA53730.17952883454277785No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC53540.17889398476494187No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA43890.1466502986801139No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT42850.1431753314751169No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT37130.12406301184763337No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT36460.12182433105210648No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG35420.11834936384710946No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTA34480.1152085281041314No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC34260.11447343888768974No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT34020.11367152337884427No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA33070.11049727448966432No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT30900.10324662176385326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC2000.047.12407145
CGGTGCA16100.045.0301632
ACGAGGA2200.042.8400574
TGACGCC3900.040.896626145
CGAGGAT2450.038.4686245
CGACGAA7000.036.249283145
ACGTGCA15350.035.8950542
GTCTCGC1656.024675E-835.1508181
GTACAAG4600.034.6732251
CAAGAGG81550.034.671298
TCTCGGG8650.034.36348145
CACGAGG4800.033.2285083
GCACGCA69550.030.229452
CACAGCG6200.029.2332923
CGTATCC6450.029.224226145
CGTCCAG1504.402196E-528.9994245
CACGCAC73500.028.6048763
CAGCCCG77150.028.1912775
GATTGGG75600.027.6185022
ACAGCAT36250.027.1994613
Produced by FastQC (version 0.11.5)