FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684824
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT38520.22862922180595718No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC34570.20518463649615626No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC30830.18298647217750935No Hit
GTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT24670.14642478977032616No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT22600.13413864000037987No Hit
GAAACATTGTCTCTGGAGACCATATAAACAACAAAGATAGCTATCATCAG18890.11211853582332636No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA18700.11099082159323466No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT18070.10725155861977276No Hit
AAGCAGAGCAGAGGAGGAGAGGGTTCCCAGGGCTGTGTGTGTTAAGTGGG17910.10630190453127447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA52150.097.8701254
TAGTAAC56550.090.6397252
CGGTGCA4250.086.998752
TAACAAG60150.085.0943765
TGCGGCG554.3966793E-879.089785
ACAAGAG67150.076.223787
AGTAACA68500.074.933233
GCACGAA300.00193608372.49896145
CGGTGCG1550.065.482932
GTAGTAA82600.063.897391
CGACGAA1850.062.701805145
CAAGAGC58550.060.673778
AAGAGCG11650.058.4987539
AAACACG18600.058.4669085
AACAAGA89650.057.4169126
CAGCGCA2100.055.238949
GTGCGGA953.1415766E-853.4202844
TAGAAAC29050.051.161062
GAAACAC22250.048.8757064
CCTCGTA752.5511023E-548.3340769
Produced by FastQC (version 0.11.5)