FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592111
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCT124980.48215527807258257No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT118470.45704061284412595No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA104530.4032620516636826No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT100200.38655752010619915No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA85950.33158302248630556No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC76390.29470188583745066No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC67580.26071414380016905No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC62620.241579160768964No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA61650.2378370370713291No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT60760.23440354213226208No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG56180.21673454570425416No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG52930.20419650238743636No Hit
GTAGAAACAACAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAA52800.2036949806547636No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC51460.19852544894875257No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT50390.19439753930290796No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA49750.19192850923436536No Hit
GTCTATTGGAATTGAAGGGTTTGAGCCATACTGTATGAACAGTTCCTCAA47370.1827468036669726No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG46050.1776544291506035No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT44890.17317931215137006No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG43570.16808693763500096No Hit
CATAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAA43500.16781688747125412No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA41770.16114279056722494No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGC41330.1594453323951019No Hit
CTCCAATGTTGCTTTGCAACTCTTCTGCCAGCTTTTGGACGTCTTCTCCT39040.15061083418109797No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTG38900.15007073385360428No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC38510.14856616865558614No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT38380.1480646469229134No Hit
TAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAACA38120.147061603457568No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC37080.14304942959618627No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG36930.1424707506738716No Hit
GTACTTCCCTCATTGTTTCTTTGGCTTGGATTTCTTTTTGGTAACTGTGT36740.141737757372273No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG36320.14011745638979195No Hit
ACATTGGAGTGCTGAGATCTCTTGGGGCAAGTCAAAAGAATGGGGAAGGG36270.13992456341568707No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT35320.13625959690769415No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAATTGAATTT34670.13375198824433057No Hit
CCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGA33610.1296626571933069No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA33280.12838956356421466No Hit
GTAGTAACAAGAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAA32320.12468601846140077No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA31940.12322003185820361No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG31800.12267993153070993No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT31520.12159973087572253No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGCAACT31430.12125252352233372No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT31250.12055810881555612No Hit
CTCTTGGGGCAAGTCAAAAGAATGGGGAAGGGATTGCAAAGGATGTAATG31230.12048095162591416No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCTCAT30660.11828197172111841No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA30630.1181662359366555No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC30510.11770329279880375No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT29980.11565862727329192No Hit
TAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAATGCAGA29910.11538857710954509No Hit
GATTCTGGCTGAGAGAAAAATGAGAAGATGTGTGAGCTTTCATGAAGCAT29740.11473274099758844No Hit
CCAACAGACGGATTCATAAATAGGAGTACTTTCATCATTTTTACTCCAAT29480.11372969753224303No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGTAAG29360.1132667543943913No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG29350.1132281757995703No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT29180.1125723396876137No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA29160.11249518249797172No Hit
CTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATCTTA28630.11045051697245989No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC28230.10890737317962076No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT28060.10825153706766416No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG27930.10775001533499144No Hit
AAACAACAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAAAATG27760.10709417922303481No Hit
AAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAACT27760.10709417922303481No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27560.10632260732661525No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA27370.10558961402501667No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA27250.10512667088716494No Hit
TTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGA27200.10493377791306005No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA26920.10385357725807268No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC26570.10250332643933845No Hit
GGCTTGGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGA26070.10057439669828953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA38950.091.9506454
TAACAAG42000.084.2375645
AGTAACA45250.079.789563
TAGTAAC45300.079.541442
CTCGCTC3550.077.605153
GTCTCGC3750.075.399531
CGGTGCA4150.075.120012
AGCACGC3550.071.4784241
GTAGTAA57550.063.4922181
CGACGAA3450.063.04308145
CAACGCA24450.062.269559
AGCGAAG350.003567232562.1424672
ACAACGC25550.059.8724258
GCACGCA4250.057.9996382
ACGATAA2750.057.999638145
TCGCTCT4750.057.9996384
TCTCGGG6850.056.09454145
AACAACG27900.054.829417
GGGCCGC553.2200594E-452.726944145
AACAAGA71500.049.1780136
Produced by FastQC (version 0.11.5)