FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533691
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAAGAAGGCTGGAAGGTATTTGGCAGGGGGGCGACAGAAATAGAA89380.3527659844866639No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC62720.24754399806448377No Hit
GTAGTAACAAGAGCTTGCTTAAAAGATACAATACAGAATTCTTAACAGAG59030.23298026475998848No Hit
CTTCTACACCATATGAATTAAAAGCACATCAGTGAGAAAATTAACTGGAA42450.1675421351696004No Hit
GTAGTAACAAGAGCAATCACTTCTCATTTCTCACTGTTTCTGTGCATAAG40750.16083255614042913No Hit
AAATAAGGCAACAATGAGAAACGTTACCTACAAGATGGCAGAAGACCAGA39890.1574382985139072No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC38790.153096806200914No Hit
AACAACAGCTGTGTAGGGTAGCTAGCAGTTAGTGTTGTACAGAAGACAGA38350.15136020927571672No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA37770.14907105878341126No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG37750.14899212255953864No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT37650.14859744144017561No Hit
GTGTAGGGTAGCTAGCAGTTAGTGTTGTACAGAAGACAGATATTTGTGCA35170.1388093496799728No Hit
ACTTCTACACCATATGAATTAAAAGCACATCAGTGAGAAAATTAACTGGA33820.1334811545685721No Hit
GTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATA33560.13245498365822825No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG33000.13024476938979537No Hit
ATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATT33000.13024476938979537No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT31530.12444295693515903No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA31530.12444295693515903No Hit
GTATAGAATTGCTGGGCTGCAAAGATTCAATTAAGTCACACTGAGCACTT31370.12381146714417819No Hit
GTGTAGAAGTAGGCTGGAAGGTATTTGGCAGGGGGGCGACAGAAATAGAA31020.12243008322640764No Hit
TCTATGTACCAAGCACTCTGCCAAGTGCTCAGTGTGACTTAATTGAATCT30750.1213644442041275No Hit
GTAGTAACAAGAGCGAAACTCCATCAAAAAGAAAAAAAAAGAAAGAGAGA30580.12069348630121036No Hit
CTTTTACACCATATGAATTAAAAGCACATCAGTGAGAAAATTAACTGGAA29850.11781231412986036No Hit
ATTCTATACATTGGGTTATTGTCTCCATTTCACATATCAGCACACTGAGG28310.1117342248916699No Hit
TATATATTCTAGCTCTGATAAAGTTACAAGATCAATTATATGTACTACAG28090.11086592642907127No Hit
GAAGTAGGCTGGAAGGTATTTGGCAGGGGGGCGACAGAAATAGAACCATG27610.10897145705612878No Hit
TTACTAAACTGTGTGGAAGGCTTCCAGAGATTTAATAGTCTACCTCGAGG27210.10739273257867672No Hit
ATATATTTCTTAATCTGTAATGATAATGTGATTTCCAAGCATTTCATCAT26610.10502464586249861No Hit
ACAACAGCTGTGTAGGGTAGCTAGCAGTTAGTGTTGTACAGAAGACAGAT26000.10261709103438423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA64300.0105.310081
GTAACAA68500.098.006414
TAACAAG78650.085.174055
TAGTAAC80750.083.4976962
AGTAACA84050.080.133123
GCGACGG300.001936156172.499344145
CGAGCGA300.001936156172.4993445
AAGAGCG28450.067.020489
GCACGCA5600.063.4369162
CAAGAGC104050.062.9883778
AACAAGA108250.062.0179066
ACAAGAG109800.061.0764057
AACACGA19000.056.8547481
AGCACGC6150.056.584851
TGACGCC2950.054.067303145
AAACACG19450.052.557365
ACAACGC5450.051.880268
CGACGAA2400.051.3537145
TCTCGGG6450.050.580933145
CTACACC14350.049.511744
Produced by FastQC (version 0.11.5)