FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769569
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA92420.33369813136989906No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG58540.2113686281150605No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG58050.20959939976220127No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG50930.18389142859412422No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT50650.18288044096391895No Hit
CACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG49130.17739222239994745No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC45870.1656214378482717No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT42960.15511438783435255No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAATCTCAGTAAGAACAATT40400.14587107235819002No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA38640.13951629296832826No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC37830.13659165018094874No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT34570.124820865629273No Hit
ACACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATA32600.1177078455167573No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC32540.11749120531028474No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT29970.10821178313304344No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG29310.10582874086184528No Hit
AACACGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC28910.10438447281869491No Hit
ACACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATA28110.1014959367323941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA96200.0113.4207464
GTAGTAA99550.0110.4779051
TAGTAAC99100.0110.394312
GCACGCA28200.0109.0053562
AGCACGC29900.0102.8077241
AGTAACA107400.0101.86293
TAACAAG108900.0100.326695
CGCACTT32850.096.4444355
CACGCAC33850.091.453493
ACGCACT35200.090.4175954
AAACACG41650.077.981965
ACAAGAG141200.077.171237
CGGTGCA9600.077.030022
GCGGTGC18100.076.904851
ACGTGCA23650.076.33072
GTCTCGC19800.075.428091
AACAAGA145400.074.991936
GAAACAC43950.073.406114
CAAGAGC138400.073.336988
CTCGCTC20500.073.2061543
Produced by FastQC (version 0.11.5)