FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817153
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT205020.7277560004728177No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC115050.40839102455564175No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT103200.36632728147885474No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC59480.21113514246475076No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA58330.20705300706067437No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA58130.2063430704686611No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG56240.19963416967413555No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT53370.18944657957874492No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA42240.14993860823320565No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA41160.1461049506363339No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38330.13605934785934592No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG37610.13350357612809813No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT37560.1333260919800948No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG35290.125268311660744No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34490.12242856529269087No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG33340.11834642988861449No Hit
GTAAGAAGTACAGTAAAAATGAATTTAATGCGATTTTTCTACCTCCAAAA33310.11823993939981252No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT32750.1162521169421753No Hit
GTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTCTTAGTGGAG32470.11525820571335671No Hit
GGGTCTTTTTGCTGTGTAACGGTTATCTCTACAAGCACATTCTATGGTTT30750.10915275102204246No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC30160.10705843807560327No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC30110.10688095392759996No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT29530.10482213781076143No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT29220.10372173609314085No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA28760.10208888193151029No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC28730.10198239144270829No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGG100.0070916107144.999531
AGCACGC29750.0129.890341
CACGCAC30350.0127.561363
GCACGCA30350.0127.322482
GAAACAC16600.0122.72554
CGGTGCA5050.0116.2867362
AAACACG18000.0112.7774055
GTAACAA57750.0106.9606864
CGCACTT38650.0102.41885
ACGCACT38600.0102.175824
AACACGT5400.096.666356
CGGAGAT301.5999929E-596.666349
GCGGTGC11200.090.62471
TAACAAG68350.090.372795
ACGTGCA11350.086.8719562
TGACGCC1200.084.58305145
AGCCACC950.083.94708145
ACACGTG6500.080.3074347
AAGCACG3000.079.749731
TAGTAAC81200.076.6961752
Produced by FastQC (version 0.11.5)