FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520374
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT156590.6212966805720104No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC86790.3443536554495483No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC75280.2986858299601567No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA65790.26103268800582774No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG58280.23123552298190664No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT42090.1669990247479144No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT40890.1622378266082732No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA40220.15957949098030688No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT36260.143867537119491No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT35990.14279626753807173No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA35490.1408124349798879No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAAT30700.12180731907248687No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC30690.12176764242132318No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA30200.11982348651430304No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG29810.11827609711891965No Hit
GTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAATGCAAG28970.11494325842117083No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA28760.11411004874673361No Hit
GTAAGAAGTACAATAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA27300.10831725767683686No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT26890.10669051497912611No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT26280.10427023925814186No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA26240.10411153265348715No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG26200.10395282604883244No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT26100.10355605953719567No Hit
GCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT25930.10288155646741316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA27100.0122.259352
AGCACGC27550.0120.262371
CACGCAC27900.0118.75373
GCGAAGC352.7630813E-7103.570743
CGGTGCA6150.0100.202592
GCGGTGC11450.081.680691
AGCGAAG451.2334804E-680.555022
CGCACCC502.3073499E-672.49952145
TAACAAG78550.069.315275
ACGTGCA9250.068.972522
CAAGAGG34400.067.862938
CGGATGC651.3989848E-766.922643
TGACGCC1900.064.868145
GCCGACC902.8194336E-1064.444016145
GCACTTT55650.060.3185586
GCGGAGC11150.059.1700171
CGGTGCG2850.058.5083852
TCCGGAT753.7636892E-757.999621
CGCACTT60350.057.5431145
ACGCACT61050.057.0020834
Produced by FastQC (version 0.11.5)