FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554922
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA199250.5604904974005055No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCG152570.42917959943987516No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTA123780.34819329369251983No Hit
GGGCTGTTGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCC117570.330724555981819No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTA116520.32777090467807735No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCC99290.2793028932842971No Hit
ACAACAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAA94930.26703820787066496No Hit
GGCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTACTAATAAC84660.2381486851188296No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACAGCCGGGTTATTAG82540.23218512248651307No Hit
CAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAG78380.22048303732121266No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTGTTGTTTCTACTAATAACCCG78240.22008921714738047No Hit
GTAGTAACAAGAGCCGGGTTATTAGTAGAAACACGTGCGGGTTATTAGTA76990.216572965595307No Hit
CCCGGAATCCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAA76930.21640418552080748No Hit
GGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGGATT74260.20889347220557863No Hit
GTAGAAACACGTGCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGT74110.2084715220193298No Hit
ACAACAGCCCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAA73300.2061929910135862No Hit
GGCTGTTGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCG71260.2004544684806024No Hit
CACGTGCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACA70340.19786650733827635No Hit
GTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTA70260.19764146723894363No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTA65930.18546117186256125No Hit
TGTTTCTACTAATAACCCGGAATCCTCTTGTTACTACTAATAACCCGGGC64330.18096036987590727No Hit
GGGCTGTTGTTTCTACTAATAACCCGGGCTGTTGTTTCTACTAATAACCC63840.1795819992674945No Hit
TGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCGGGCTCT59550.16751422394077845No Hit
GGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCG58900.16568577313370025No Hit
CCCGGGCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAA58660.16501065283570218No Hit
GTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTA52590.14793573529883355No Hit
GGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGC49250.13854031115169335No Hit
GGGCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTACTAATAA48060.13519283967411944No Hit
ACAACAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTA47180.13271739858145973No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTGTTGTTTCTACTAATAACCC45590.12824472660722233No Hit
GCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCGG44720.1257974155269792No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCCG43100.12124035351549205No Hit
ACAACAGCCCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTA43000.12095905339132616No Hit
GGGCTCTTGTTACTACTAATAACCCGCACGTGTTTCTACTAATAACCCGG40750.11462980059759398No Hit
ACAACGCACTTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTA40680.11443289051067788No Hit
CAACGCACTTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAG40490.11389842027476271No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACGCACTTCCGGGTTA40070.11271695975326604No Hit
CAAGAGGATTCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAG39850.1120980994801011No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCGGGTTATTAG39120.11004460857369024No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCGGGTTATTAG37950.10675339712094949No Hit
CCCGCACGTGTTTCTACTAATAACCCGGCTCTTGTTACTACTAATAACCC37580.10571258666153575No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTA37370.1051218564007874No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCC37290.10489681630145471No Hit
GGGCTCGTGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCC35930.1010711346127988No Hit
ACAACAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAA35730.10050853436446706No Hit
GGTTATTAGTAGAAACACGTGCGGGTTATTAGTAGTAACAAGAGCCCGGG35610.10017097421546801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6750.076.257641
CTCGCTC7050.070.955933
TCGCTCT7800.064.133254
TCTCGCT9750.051.30662
CTTAGGG9850.046.370216145
CTGTCGT10200.045.489879
ACAGGAT8450.044.6144334
TCTCGGG12700.044.527237145
TCGCCGG24350.040.49252145
AGACAGT13000.039.595316
GATTGGG90150.038.4406662
GCACGCA50000.038.4241832
CAGGATT9950.037.8886385
CGGTGCA13400.037.8723262
GTACAAG10050.037.5116351
TCGTCCA7050.037.020494
CCTTAGA13800.036.2492262
CTTAGAC13800.035.1985243
GCACCTA4750.035.104516
GGATTGG102050.034.59751
Produced by FastQC (version 0.11.5)