FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684151
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA93950.35001756607582807No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG67740.25237030256494514No Hit
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA63460.23642485091188983No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA53510.1993554013913524No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT43330.16142907012310412No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC38440.14321101905220682No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT37370.139224656138943No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA36820.13717559109006908No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC30160.1123632761346139No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT28940.1078180772989299No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA106350.0124.069091
TAGTAAC109150.0120.687112
AGCACGC41650.0116.4503561
GCACGCA41750.0115.824132
GTAACAA116750.0112.706614
AGTAACA120700.0109.318533
TAACAAG124700.0105.5212255
CACGCAC47900.0101.104533
CGCACTT51100.099.1712955
ACGCACT54000.096.9333344
ACAAGAG151200.087.0766757
GAAACAC44050.085.7475054
AACAAGA156300.084.328176
CGGTGCA8450.083.223252
AAACACG45400.083.197755
CAAGAGC149900.082.4632648
GAAGCGG12700.076.49461
AAGAGCC86750.072.70899
GCGGTGC19950.071.226711
ACGTGCA25550.068.951712
Produced by FastQC (version 0.11.5)