FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3949708
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT190030.4811241742427541No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC178770.45261573766972146No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT145240.3677233861338611No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT91880.23262479150357443No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC90850.2300170037886345No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT88100.22305446377301816No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT77270.19563471527515452No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT64300.16279684472877487No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT59850.15153018906714116No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT55090.1394786652582925No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA53110.13446563644704876No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG51580.1305919323656331No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCT49930.1264144083562633No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA48980.12400916725995947No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT48220.1220849743829164No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC45700.11570475589587888No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA44790.11340078810889312No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT44160.11180573348713373No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC43310.10965367566412505No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA42250.10696993296719656No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG41720.10562806161873231No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC41420.1048685117988469No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA40640.10289368226714482No Hit
GAGTAATATTTACTAAATGCTTGATGCACTATGTATTTGGAAATGCCCAA39680.10046312284351147No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTACG100.007091818144.998841
GTAACAA88100.0130.515414
AGCACGC29100.0130.05051
GCACGCA29050.0130.024772
CACGCAC29400.0128.476873
TAACAAG96400.0118.751645
CGGTGCA6300.0115.078442
GAAACAC30050.0108.326924
CGCACTT36150.0105.8912665
ACGCACT36500.0104.875884
AAACACG31700.0103.145865
TAGTAAC123500.093.808972
TTCGGAA1250.092.79926145
TAGAAAC38550.090.8358462
ACGTGCA20500.088.767582
AGTAACA130950.088.471993
GACCCCC1600.081.56185145
ACAAGAG154450.074.5882647
CACGTGC30000.072.982751
AACACGA37600.070.571246
Produced by FastQC (version 0.11.5)