FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2747799
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA71560.26042661781302057No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA67450.24546919188776184No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT65820.2395371713869901No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC62050.2258170994312175No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT54180.1971759943139946No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG49790.18119957100210024No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG48350.1759590130136884No Hit
CACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG47030.1711551681909776No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT46430.16897160236247266No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG36520.132906373428333No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC36460.1326880168454825No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT29290.10659440519484868No Hit
ACACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATA28110.10230005906545565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC28250.0115.485751
GCACGCA30300.0107.193822
GTAACAA102100.0104.666114
TAACAAG105400.0101.457885
TAGTAAC105950.0100.794342
CGCACTT34800.098.332515
GTAGTAA112000.096.126421
CACGCAC33900.095.81043
AGTAACA115950.092.351533
ACGCACT37600.091.202694
AAACACG36700.082.969335
CGGTGCA8400.081.9933552
GAAACAC38050.080.216144
ACAAGAG134850.079.461697
AACAAGA141400.075.985926
GTCTCGC12000.075.52021
GCGGTGC15100.074.419911
CTCGCTC12450.071.043583
CAAGAGC137100.069.7495968
CGCTCTG12700.069.074225
Produced by FastQC (version 0.11.5)