FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454012
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC123640.5038280171409105No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT86360.3519135195752914No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC78330.31919159319514334No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT58830.23972987907149595No Hit
TTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAA56900.23186520685310424No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT46080.1877741429137266No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT43850.17868698278574027No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA38630.15741569315879464No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG36160.14735054270313266No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA35610.14510931486887596No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC34360.14001561524556522No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA34320.13985261685761927No Hit
ATATCCGAATATAGTAAGTATGATAATGAAGCTGACACATATAGTGATAA33530.13663339869568691No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA32540.1325991885940248No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC31600.12876872647729515No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT27780.11320238042845755No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC27600.11246888768270082No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC26950.10982016387857924No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA25700.1047264642552685No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA25590.10427821868841716No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC25170.10256673561498478No Hit
ATTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACA25100.10228148843607937No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG100.007091764144.998117
AGCACGC7700.0132.758011
GTAACAA59250.0127.745164
GCACGCA8000.0126.8733442
CACGCAC8450.0119.25883
GGTGCAT7300.0116.197113
CGGTGCA7700.0113.927092
GAAACAC17300.0110.2153244
TAACAAG69350.0109.0360645
CGCACTT9700.0105.3852165
ACGCACT9900.0104.7208564
AAACACG18950.099.470995
TAGAAAC21050.098.502272
TAGTAAC82750.092.167982
ACAAGAG85850.087.9109047
ACGTGCA12450.087.348262
CTGTCGT1300.083.652751
CTCGCTC703.092282E-1182.856063
AGTAACA92200.082.79993
CCACTAT2650.082.0743948
Produced by FastQC (version 0.11.5)