FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873906
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC58400.20320775975275462No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT50250.17484914259547807No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA48010.16705487235838612No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC41150.14318491975729197No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA40700.14161910654001905No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA32940.11461752750437906No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC32010.111381513522015No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC30600.10647529877455977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC28650.0106.534541
GCACGCA29350.0103.746662
TAGTAAC117000.099.3920062
GTAGTAA119000.098.5744861
GTAACAA118100.098.036544
AGTAACA126250.092.224673
TAACAAG132050.087.569985
CACGCAC35900.085.221853
CGCACTT36900.083.698215
TCCGTCG353.4385746E-582.856188
GTCTCGC10800.081.225911
ACGCACT42150.078.6052554
ACAAGAG150400.076.307297
CGGTGCA6450.069.689112
CTCGCTC12600.069.622213
AACAAGA167100.068.681136
CGCTCTG12250.068.652265
AAGAGCG18150.067.905559
GCGGTGC14500.067.4992141
ACGTGCA17300.067.051242
Produced by FastQC (version 0.11.5)