FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365739
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC50650.37086148964040716No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT36690.2686457661383324No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC32330.23672165765201114No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT27160.1988666941487356No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT26350.19293583913178142No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24470.17917039785786304No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA24170.17697378488862073No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT18440.13501847717609294No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACAATGATGGT15970.11693303039599806No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG14250.1043391160390089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC12100.0115.639123
GCACGCA12250.0114.223132
AGCACGC12700.0109.6049961
CGCACTT14050.099.073565
GTAACAA42450.095.982374
ACGCACT15550.093.246494
TAACAAG43650.093.177595
AAACACG14000.086.998965
GTCTCGC3950.086.26481
GTAGTAA47950.085.426511
TAGTAAC47750.084.7215042
CGGTGCA3250.082.537482
GAAACAC14800.081.8064654
AGTAACA51200.080.003933
CTCGCTC4200.077.677653
ACGTGCA8250.077.332422
GCGGTGC8000.075.217861
CCACTAT13700.074.0867168
AACACGT5200.073.893356
ACAAGAG55950.073.211827
Produced by FastQC (version 0.11.5)