FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939735
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC83180.2829506741253888No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC82190.2795830236398859No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA77050.2620984544525272No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA52560.17879162577579272No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA46430.15793940610293106No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA45670.15535413906355505No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA43020.14633972109730978No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA39510.13439986937598117No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT39080.1329371524984395No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA38120.12967155202764874No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG37840.12871908522366812No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT37430.1273244016892679No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT36580.12443298460575528No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA36200.12314035108606729No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA34550.1175276002768957No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG34110.11603086672778329No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT34030.1157587333552174No Hit
GTCTCGCTCTGTCACCCAGGCTGGAGTGCTGTGGCGCTATCTCGGCTCAC33910.11535053329636855No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT33530.11405789977668056No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG30830.10487339845258162No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA30490.10371683161917657No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA100.0070917113144.998957
CGGTGCA4600.094.564532
GTAACAA95900.091.928434
TAGTAAC101800.086.742992
GTCTCGC10250.086.292061
TTCGGAA703.092282E-1182.85654145
AGTAACA113100.078.3968663
TAACAAG115550.076.4209065
CGCTCTG11850.075.8644265
GCACGCA5100.072.499472
CTGTCGC6250.068.43959
TCTCGCT12950.068.300662
ACAAGAG129150.068.0928047
AGCACGC6150.064.836931
CACGCAC5850.063.204673
ACTTCGC702.336219E-762.1424038
CGCACTG3250.060.230335
AACAAGA154300.057.0880436
GTAGTAA162750.056.2622641
CGGAGCA9050.056.076942
Produced by FastQC (version 0.11.5)