FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547319
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC79850.5160538970955568No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT62240.402244139702285No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC52050.33638829485064164No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT41140.26587924015668396No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT34070.22018730462173608No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC27730.1792132068435791No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT19720.12744624734783194No Hit
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT19310.12479650285429185No Hit
GAATAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCT19090.1233746887358069No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC18050.11665338563024172No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC17240.11141852455763808No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG17210.11122464081420831No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT17150.11083687332734879No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT17080.11038447792601268No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA17000.10986745461019995No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA16040.1036631748204475No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGCG100.0070909695145.00182145
GTAACAA54650.0123.373584
TAACAAG56500.0119.3339165
AGCACGC7350.0111.460371
GCACGCA7850.0105.2845462
TAGTAAC64750.0104.577082
CGGTGCA4900.0100.6102452
AGTAACA67950.099.43883
ACAAGAG69900.096.4573067
CGCACTT9200.094.563355
AAACACG14950.091.6536945
ACGCACT10100.091.161554
TTCGGAA406.1186074E-790.62613145
CACGCAC9150.090.326093
GTCTCGC10500.089.7601241
GAAACAC15600.088.299524
CTCGCTC10450.087.4145363
TGACGCC2800.080.26886145
CAAGAGC62100.080.203728
AACAAGA84650.079.6499256
Produced by FastQC (version 0.11.5)