FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827260
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC100730.35628134660413263No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA70100.24794323832969023No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC69850.2470589899761607No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA60150.21275015385921353No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT51910.18360532812687902No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT51530.18226127062951408No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT34960.12365328975757446No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA28590.10112264170964112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA111000.0118.415774
TAGTAAC116650.0113.0531542
AGCACGC23800.0112.404621
TAACAAG117450.0112.036195
GCACGCA24150.0110.775562
AGTAACA125400.0105.164673
CGCACTT28900.094.3245545
ACGCACT30650.091.540914
ACAAGAG145100.090.63687
GTAGTAA150300.088.851631
CACGCAC30500.088.663273
CGGTGCA10250.083.462782
AAACACG37700.080.19175
CAAGAGC137700.079.238698
AACAAGA166850.079.125866
ACGTGCA20950.074.748842
GAAACAC41000.073.737244
GAAGCGG10950.068.8579251
TAGAAAC56450.064.0872042
GTCTCGC13550.063.6711041
Produced by FastQC (version 0.11.5)