FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799344
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA68590.24502169079612937No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT66320.23691264810612772No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA57370.20494087186140753No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT56440.2016186649443584No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG53520.19118764967792454No Hit
GGATAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA53090.1896515755119771No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA49470.1767199743940009No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC48370.17279048234157718No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA44750.15985888122360095No Hit
CTATTATCCAGGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT44210.15792985785241115No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT44170.157786967232323No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC38430.13728216324967563No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG37310.13328122588720787No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT34130.12192142159020113No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC33860.12095690990460621No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA32120.11474116793077234No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG31580.11281214455958254No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT31340.11195480083905371No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA30280.10816819940671814No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29770.10634634400059442No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT28870.10313130504861139No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT28420.10152378557261987No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCTCCATTCTTTTGG28110.10041638326693683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA11050.0120.06692
AGCACGC11400.0116.380631
CACGCAC12700.0104.467653
CGGTGCA3950.0102.783982
CAGCGCA451.092485E-896.6658869
GCGGTGC7350.088.774791
TGACGCC2500.086.99929145
GCCGACC703.092282E-1182.85647145
GAAGCGG6300.081.705691
GTAACAA55200.075.520224
ATTCGGG406.6655484E-572.49941145
TAACAAG61200.068.116275
CCAGCGC651.3990575E-766.922538
GGATCCC350.003567288462.142353145
AACAACG19100.061.1120727
AAACACG18700.058.1546065
ACAACGC20400.056.8622868
CAACGCA20800.056.1173329
GAAACAC19450.055.912144
AGCGGAG7750.055.1930963
Produced by FastQC (version 0.11.5)