FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2511830
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT85450.3401902198795301No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC51990.206980567952449No Hit
TAACCGTTACACAGCAAAAAGACCCTTTGTCAAATTAAATGTGGAGACTG48840.194439910344251No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC46770.18619890677314946No Hit
TAACCGTTACACAGCAAAAAGACCTTTTGTCAAATTAAACGTGGAGACTG42390.16876142095603605No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT42320.16848273967585387No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT40240.16020192449329773No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA39600.15765398136020353No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT35730.14224688772727453No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG34210.1361955227861758No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA33980.13527985572272008No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAAT29030.11557310805269466No Hit
GTCTCTTTTCCACCATCATGTACCATCTCTATCCCAATACAGGGGACATC28920.11513518032669409No Hit
GACTCAACCATTCCTCCATTACAGAGCCATATCAACACCTGTGACAGTGT28830.11477687582360271No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG27470.10936249666577753No Hit
GTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAATGCAAG26650.10609794452650059No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC26580.10581926324631842No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA26310.10474434973704431No Hit
GTACCTGTTGTCTCTACAGGCACATTCTATGGTTTTATTGCTGGCAAATC25940.10327132011322421No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA25250.10052431892285704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA24150.080.1541442
CGGTGCA7400.079.356982
CACGCAC27250.071.833853
AGCACGC30000.069.840681
CGAGCTA1750.066.2847755
ACGTGCA10700.054.88242
GCGGTGC19500.053.5377041
GTAACAA151550.052.143774
TAACAAG152000.051.846315
ACCGTTA26150.050.1809353
TAACCGT26400.049.9803541
TAGTAAC161550.049.454582
GTAGTAA167250.048.419351
CGTTACA27350.047.7141345
AGTAACA167350.047.653943
GTTACAC27450.047.27626
CCGTTAC27850.047.1178254
AACCGTT28800.046.0670552
CTTAACC6250.045.239363
CAAGAGG44250.044.2366648
Produced by FastQC (version 0.11.5)