FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867272
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA154930.4006183169945119No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT120680.31205459559089715No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC108510.2805853842191602No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT79690.20606256813588494No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT62890.16262109311163012No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA58630.15160557623047977No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG56230.14539965122701481No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC56180.14527036112277594No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC54130.13996946684898295No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC53740.1389610040359199No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA51120.13218620257380395No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC49410.12776448100883517No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC48790.1261612837162734No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA48390.12512696288236255No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG48100.12437708027777719No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA48050.12424779017353835No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT47120.12184299423469568No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA46970.1214551239219791No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT46270.11964506246263516No Hit
GTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA44140.11413730402205999No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT42430.1097155824570912No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT41290.10676776808044533No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG40990.10599202745501222No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC40930.1058368793299256No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA40040.103535515474474No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA39380.10182888609852113No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT38830.10040669495189374No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCC100.0070916465144.99997145
GCACGCA12600.0115.077862
AGCACGC12750.0113.7241
CGGTGCA7100.0107.216912
CACGCAC13550.0106.4746253
GTAACAA98950.097.813264
TAACAAG100450.096.78575
GAAACAC24700.092.164794
AAACACG26300.087.109135
GCGGTGC19200.080.428631
AACACGT8750.078.713256
CGCACCC651.5661499E-978.07691145
TAGTAAC127100.076.948242
ACGTGCA9450.074.4170152
AGTAACA134150.072.68823
GTAGTAA137050.072.63131
TACGCTC300.001936165272.499985145
CGCACTT20000.072.136565
ACGCACT20700.071.09814
AAGAGCC45700.065.995619
Produced by FastQC (version 0.11.5)