FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874432
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT123680.4302763119809409No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC119070.4142383608309398No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT101040.3515129249883107No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA61250.2130855765591254No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG53610.18650641239730145No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC46620.16218856455814573No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT39020.1357485583238706No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA36810.12806008282679848No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG32290.1123352370137822No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA31580.10986518379979071No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA30830.10725597265825039No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT30000.10436844566161245No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG29040.10102865540044086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC22000.0123.907411
GCACGCA22700.0120.086472
CACGCAC23900.0114.663723
GTAACAA67400.0104.0156564
CGGTGCA6400.098.553362
AAACACG18550.097.7075655
GAAACAC18850.096.5371554
CGCACTT28700.096.4969565
ACGCACT28900.096.330874
TAACAAG73450.095.250585
GCACGCG259.389057E-486.998822
AACACGT5900.081.100596
GCGGTGC12500.080.6189041
ACGTGCA10950.080.113072
TAGTAAC89000.079.5862
AGTAACA95850.073.822683
GTAGTAA104850.068.730581
GCCGACC1500.067.66693145
ACAACGC6850.064.561178
GTCTCGC4000.063.4366381
Produced by FastQC (version 0.11.5)