FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718546
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC96980.35673481338921614No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT80940.29773268504560896No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC56300.20709599911128965No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT52040.1914258577930997No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT49480.1820090592544691No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT41600.15302297625274688No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT39390.1448936306393197No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA31300.11513507588247542No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA30730.11303836683285845No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA30630.11267052313994319No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG29960.11020597039741098No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC29220.10748392706983807No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT28600.10520329617376346No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT28190.10369513703281093No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC28030.10310658712414651No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA27280.10034775942728208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGT100.0070916126144.99942145
AGCACGC8250.0121.272241
GCACGCA8300.0120.541692
CACGCAC8450.0118.4018863
GTAACAA51150.0118.0689244
CGGTGCA4900.0113.928112
TAACAAG56500.0106.8889545
GAGCGTG259.388648E-486.999647
ACAGCGT259.388648E-486.999644
GAAACAC18000.082.569114
AAACACG18000.082.569115
TAGTAAC75850.080.672052
ACGTGCA10750.077.557822
CGCACTT13900.075.1076055
ACGCACT13950.074.3186954
AGTAACA82800.073.900673
GGTGCGT4850.073.247133
AGCGTGC300.001936132672.499718
CTACTAG406.665378E-572.499711
CAGCCCG406.665378E-572.499715
Produced by FastQC (version 0.11.5)