FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912466
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC141740.4866666254644689No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC88530.30396921371785973No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT80030.27478432366249084No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT54760.18801936228611768No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT51520.17689476890030648No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA48570.16676589529285493No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA44330.1522077854299415No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA39420.13534921952736959No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA38670.13277408216954292No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG38430.1319500382150384No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG33940.11653354923284942No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA33420.11474812066475627No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT33270.11423309319319093No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT32460.11145194484673814No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC32000.1098725272672711No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA31700.10884247232414045No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT31010.1064733459549399No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC29700.10197543936993599No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA29440.10108272508588942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC10300.0123.882691
GCACGCA10500.0122.213492
CACGCAC10800.0118.8186653
GTAACAA68200.0117.6788564
CGGTGCA5150.0108.3973542
TAACAAG76450.0104.505535
GAAACAC18450.093.9153754
ACGCACT14550.090.68674
TTCGGAC406.120663E-790.62441145
AAACACG19600.088.405045
CGCACTT14750.087.490955
TAGTAAC95400.085.266752
ACGTGCA9450.084.390982
AGTAACA103100.079.250213
TTCGGAA1400.077.67807145
ACAAGAG107050.074.7005847
AACACGT6600.072.499536
TAGAAAC29000.070.9995352
CCACTAT2350.064.786818
ACACGTG7550.062.4168177
Produced by FastQC (version 0.11.5)