FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2138333
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCAAAGAAGAACAGATAGCACTGATGGAATGCCTATTA61600.28807486953622286No Hit
GTAGTAACAAGAGCCATGGTGGTTTGCAGCACCCATCAACTTGTCATCTA46510.21750587958002798No Hit
GTAGTAACAAGAGCAGCCAACACTTACGTGGTACTTATGGGCCAGAGTAC40610.1899142930497729No Hit
CACGTGCACTGTGGGGATGGTTCTGATGCTTTCAAGGCCTAGAGTCTCAC37030.17317227952802489No Hit
CACGAGCACTGTGGGGATGGTTCTGATGCTTTCAAGGCCTAGAGTCTCAC36430.17036635547410062No Hit
GTAGTAACAAGAGCTATAGTGAGGGGTCCTAAGGCCAGGAACAATACCAG35500.16601717319051804No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA31830.1488542710606814No Hit
GTTCTACACTGTGCATATAACACAACACAAGAGTAAACACAGACACGTTC29940.14001561029082No Hit
GTTTTGGGGAGTGTAAATTAGTTCAACCATTGTAGAAGACAGTGTGGTGA28170.13173813433174347No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG25790.12060796891784395No Hit
CACGTGCTCTCCCTGCCAATCGTGTCCGACTGATCTCTCAAACCCAAGCA23020.10765395286889366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC77700.0121.392142
GTAGTAA82400.0114.732031
GTAACAA86250.0109.0222554
TAACAAG93500.099.948345
AGTAACA98400.095.855383
AGCACGC9100.086.839911
AACAAGA112300.083.668036
CGAGCAC8450.076.360293
CAAGAGC124500.074.129898
CTTAGCG955.456968E-1268.6836249
ACAAGAG140500.066.823277
GTAGAAA45650.065.273271
AAACACG28050.064.357745
AAGAGCA66500.062.7964559
CGGTTAC350.003567179462.1423264
AAGAGCG13650.058.4244089
GCGGTGC6400.057.772941
ACGAGCA16000.057.5463832
TAGAAAC52350.056.6423072
GCACTGT23400.055.7687536
Produced by FastQC (version 0.11.5)