FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584818
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC168160.4690893652062671No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT141020.3933811981528769No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC65070.18151549116301025No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT60200.16793042213021692No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT58920.16435980850352794No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA50650.141290297024842No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA46980.13105267826706962No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA46390.12940684854851767No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG44750.12483199983932239No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA43370.12098243202304831No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC42480.11849973973574113No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT41830.11668653750343812No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA39700.11074481326527595No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC38040.10611417371816365No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC37940.10583521952857858No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC37850.105584160757952No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC37300.10404991271523407No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA37120.10354779517398092No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT36490.10179038377959496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA68850.0125.0973364
AGCACGC7550.0123.873491
GCACGCA7700.0121.460362
CACGCAC7900.0118.3854143
GAAACAC22050.0106.5300144
TAACAAG81300.0105.7620245
CGGTGCA7100.0104.154352
AAACACG23550.0100.052515
GGTGCAT7100.097.00653
ACGCACT10250.095.4872744
CGCACTT10400.093.412945
TAGAAAC28500.090.052132
ACGTGCA13500.088.073582
CTTAGCG502.2704626E-886.999529
TTCGGAA1100.085.68134145
TAGTAAC101950.084.90882
GAAGCGG8500.081.028951
AACACGT6250.080.039556
AGTAACA113650.076.42283
ACAAGAG116400.074.056727
Produced by FastQC (version 0.11.5)