FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777403
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG120900.6802058959054306No Hit
CAACAGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTT91910.5171027617259564No Hit
CACGAGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTT83470.4696177512921943No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG69760.39248274026768265No Hit
CACGTGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTT69330.39006348025743176No Hit
AGAGCATAGAAAACCAAGCAAATGAGAAAAACAATGTAATTAACTCCAGG61500.3460104433265838No Hit
ACAACGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTT50720.2853601574882005No Hit
ATACTAAACTGTGAATGCAGGTTTCAGCCAATATCAAACTGCTTAGGGAG46560.2619552234355405No Hit
GTAGTAACAAGAGCATAGAAAACCAAGCAAATGAGAAAAACAATGTAATT45190.2542473485191597No Hit
GTAGTAACAAGAGCTGTGCTTTTAGATGAAAATAAGTTCTATGGCTGCAT42420.23866281310428752No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA39220.22065901767916446No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA33820.1902776128992693No Hit
CAGTTATACTAAACTGTGAATGCAGGTTTCAGCCAATATCAAACTGCTTA33510.18853349521746052No Hit
ATAATATGTTGTGGGAAAGACAGTGTAAGAGAAGCTAACTCCTCTTCCAT30360.17081100909585503No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA29010.16321565790088122No Hit
AACACGAGCTGTGCTTTTAGATGAAAATAAGTTCTATGGCTGCATGGAAA28300.15922106579093204No Hit
GTGTAAGAGAAGCTAACTCCTCTTCCATTGTAGGAAGTCAACAATGTCAA28120.15820835229826888No Hit
ACAACAGCCACTCTTCTTCCTTCCGTTTTTAAGTTATAAAAAGCATTTAA27530.15488890251676182No Hit
CATATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAG26270.1477999080681196No Hit
ACATATTATCACCCCCACCCTCGACACCCACATTTCCTCTCCTATTAGTC24970.14048586617666337No Hit
ATATCAAACTGCTTAGGGAGACTAATAGGAGAGGAAATGTGGGTGTCGAG24810.13958567640540723No Hit
CTCTTACACTGTCTTTCCCACAACATATTATCACCCCCACCCTCGACACC24650.13868548663415106No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT24390.1372226782558598No Hit
ATATTATACTTACCATTGAATTTATCAGAGAAGAGTACTTAATTTCCATT23900.13446584708138784No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA23840.13412827591716678No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT23780.13379070475294574No Hit
GAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTTCTCCAAACACAC23740.1335656573101317No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG23550.132496681956765No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT23460.1319903252104334No Hit
CCAATAAAGGGCTCAACAGGAAATGGTAAACATACAGTTATACTAAACTG23080.12985237450370007No Hit
ACATACAGTTATACTAAACTGTGAATGCAGGTTTCAGCCAATATCAAACT22780.1281645186825948No Hit
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA22670.12754563821485615No Hit
GTTATACTAAACTGTGAATGCAGGTTTCAGCCAATATCAAACTGCTTAGG22270.1252951637867158No Hit
GACTTAAATAATGCTATTTCTTAATCTTGACTTGACATTGTTGACTTCCT21900.12321347494068592No Hit
GATAATATGTTGTGGGAAAGACAGTGTAAGAGAAGCTAACTCCTCTTCCA21640.1217506665623947No Hit
ATAGAAAACCAAGCAAATGAGAAAAACAATGTAATTAACTCCAGGAAAAA21540.12118804795535959No Hit
TCCATATACAGAATCTCAAATGATTCTAAAAATGGAGCCCTTAGGTAATG21230.1194439302735508No Hit
ACACGAGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCT20450.11505550513867704No Hit
ACAACAGCCAGAGTCAAAACACCTTCTTGTATTTGCCAAACATTCATTAA19690.11077960372521031No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG19630.11044203256098926No Hit
GACTAATAGGAGAGGAAATGTGGGTGTCGAGGGTGGGGGTGATAATATGT19590.11021698511817524No Hit
AACAACGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCT19070.10729136836159273No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC18980.10678501161526113No Hit
GCTTAGGGAGACTAATAGGAGAGGAAATGTGGGTGTCGAGGGTGGGGGTG18900.10633491672963308No Hit
CTACTCAACTCAGGGATCTTTAGTGATTAGCTTCATGTAAAGACTTAAAT18610.10470332276923129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA74500.097.898571
GCACGCA5450.091.788282
TGCACTA11900.088.948895
AGTAACA82750.088.050683
GCACTAA50550.086.483016
TAGTAAC85350.085.4533542
GTAACAA87850.082.361344
CGTGCAC16200.081.0024643
AGCACGC6350.079.920641
ACGTGCA21300.078.6261442
CGTGCGA755.638867E-1177.3327263
ACAGCAC18500.074.066993
AGCACTA34750.074.064185
CGAGCCG4050.073.39455
CACTAAG60850.073.035597
GTGCACT14800.072.9892964
ACTAAGA61500.072.4994358
CGAGCAC20200.071.0638053
TAACAAG102700.070.664015
CACGCAC7250.068.9994663
Produced by FastQC (version 0.11.5)