FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402593
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTAGTCATAGTATGGCTGTGGTGTATCTGTGCCACATTTTCTTAATC53040.22076148561158715No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG48430.20157388288403405No Hit
CCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTA47120.19612144046036928No Hit
ACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAG41090.17102355663235513No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT39470.16428084157408268No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA38230.1591197510356519No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG31730.13206564740678092No Hit
TCCCCTTCCTGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGT31670.1318159172194375No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC30040.12503158046327448No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT28090.11691534937461318No Hit
GGTTAGTTACATATGTATACATGTGCCATGCTGGTGTGCTGCACCCATTA27360.11387696542860151No Hit
GGGTATATACCCAAAGGATTATAAATCATGCCGCTATAAAGACACATGCA26570.11058885129524643No Hit
CACATATGCTTATTGTGGCACTATTCACAATAGCAAAGACTTGGAACCAA26440.11004776922266901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA84050.098.1606751
TAGTAAC84450.097.2664952
AGTAACA92000.089.20553
GTAACAA93400.087.4802554
TAACAAG104350.078.578395
AACAAGA110050.073.981436
CAAGAGC112850.071.182178
AAGAGCG13850.069.098129
ACAAGAG118050.068.9678657
CTCGCTC14150.068.14413
AGCACGC10900.067.843261
AAGAGCT41550.064.735869
CGCTCTG15000.064.282595
GTCTCGC16050.060.9806061
GGTTAGT10850.060.805751
TCTGTCG20300.060.713598
TCTCGCT16350.059.86172
AGTCTCG6700.058.4321632
TGACGCC9100.057.36198145
GCACGCA13150.056.786422
Produced by FastQC (version 0.11.5)