FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3923464
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA143460.3656462758419601No Hit
GTATATATGGAGCTATTTAATTCAAAGCAGTAGGATCCAGTTACTATGTG137440.35030269170304607No Hit
CTTCTACACTGCTGGTGGGAATGTAAACTGGTACAGCCACTATGGAAAAC110810.2824289964174515No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA104070.2652502992253784No Hit
CTATTATTCCAAGTGAAATAACTCAGGAATGGAAAACAAAACATCATATG96100.24493661723415838No Hit
GTATACACAACACCATATTTGTAGTCTTTTATCCCTTGCCCCCCTCCAAC93690.23879408604233401No Hit
GAATATATACAAATGGCCATCAAACATATGAAAAAATGCTCAACATCACT88690.22605024539539548No Hit
ATGGAAAACAGTGTGGAGATTCCTTAAAGAACTAAAAGTAGAACTACCAT79860.20354462281290206No Hit
AATTAGAATAGTGGTAAATGCCTAACTGGCATTCCAAAGGATGGGGTAAA75870.19337503797664513No Hit
CACATATATCACAGTTTCTTTATCCATTCATTCATTGATGGGCATTTGGG73280.18677372852153096No Hit
GTTTATAGCAGCACAATTTACAATTGCAAAATCATGAAACCAACCCAAAT72720.18534641836907387No Hit
GTATCATTCTTATGCCTTTGTGTCTTCACAGCTTGGCTCCCACATATTAG72690.18526995532519222No Hit
CTAATATGTGGGAGCCAAGCTGTGAAGACACAAAGGCATAAGAATGATAC72660.1851934922813106No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT72360.1844288618424943No Hit
AGTAGAAACAACGCACTTGCAGCCTTTCAGAAGCAGTTTTATGAAATGAG72090.1837406944475596No Hit
GTATATATATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCAGG70740.1802998574728862No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT70710.1802233944290046No Hit
CACTAATATGTGGGAGCCAAGCTGTGAAGACACAAAGGCATAAGAATGAT69800.17790401543126175No Hit
GTTATATCTTGAATTATTTAGTTAAATATCCTAACTTTTTTTTCCCATAA68160.17372403569906592No Hit
TAGTTATATCTTGAATTATTTAGTTAAATATCCTAACTTTTTTTTCCCAT65640.16730114001300891No Hit
GGAATAGACAATTCTCAAAAGAATATATACAAATGGCCATCAAACATATG65040.1657718791353763No Hit
ATTTAATTCAAAGCAGTAGGATCCAGTTACTATGTGTGCTGGATCCAACT62770.15998617548166622No Hit
ATATACAAATGGCCATCAAACATATGAAAAAATGCTCAACATCACTAATG62750.15993520011907844No Hit
CTTTTATCCCTTGCCCCCCTCCAACTCTTTCCCCCAAGTCCCAAAAGTCC62660.15970581098743356No Hit
AGATAAAGCAGTTCCTTAGTATATATGGAGCTATTTAATTCAAAGCAGTA62490.15927252040543763No Hit
GTGTATATATATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCA61150.15585717111205813No Hit
TGTGTATACTGCTTGGGTGATGGGTGCACAAAAATCTCACAAATCACCAC60200.1534358413891398No Hit
GCTATAAACACACATGTGCATATTTTTCATATAAAGACTCCTTTTCCAAT59450.15152426529209903No Hit
CATATATCACAGTTTCTTTATCCATTCATTCATTGATGGGCATTTGGGTT59290.151116462391397No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA59070.1505557334029317No Hit
GAATGATACAATGGACTTTTGGGACTTGGGGGAAAGAGTTGGAGGGGGGC59040.15047927035905007No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG58500.1491029355691807No Hit
GTTAATATGATATCAACATGTTTATAAAATTTGTGAAAAATTAACAATTG58240.1484402558555399No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG57550.1466816058462624No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG57530.1466306304836746No Hit
GTGTAGAAGTGTTCCCTGATCACCAAATTCAAGCCAACCTCTACTGTTTT57490.14652867975849912No Hit
ATTCTAATTAAGTCTTCAAACCTAAACCAAATTTTACTTAGTAGGCACTT57480.14650319207720525No Hit
ATATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCAGGAATGGA55360.1410998036429033No Hit
AATATATACAAATGGCCATCAAACATATGAAAAAATGCTCAACATCACTA55050.1403096855227931No Hit
CATATTAACAGGTAGAAATTGATCATGGTAGAAAATATTTTACATCATGG54330.138474572469634No Hit
GAATATAGTTATATCTTGAATTATTTAGTTAAATATCCTAACTTTTTTTT53730.13694531159200135No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT53500.13635909492224219No Hit
GGATACAGGTGGTATTTGCTTACATGAGTAAATTCTTCAGTGGTGATTTG51500.13126155866346678No Hit
ATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCAGGAATGGAAA50900.12973229778583414No Hit
ATATATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCAGGAATG49900.12718352965644644No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC49670.12659731298668728No Hit
GGTTTAATGAATATAGTTATATCTTGAATTATTTAGTTAAATATCCTAAC49610.126444386898924No Hit
GTTACTATGTGTGCTGGATCCAACTCATATCAGTTTGCAAGAGTCAATTG48260.12300354992425061No Hit
GTATGAAACTGCTTTATCAATAAATGCAAACAAAAATGTATGCTGGCAAC48220.1229015991990751No Hit
CCATAATGTGATACCACCTTCCTCCTGCAAGAATAGCCGTAATCAAAAAA45770.11665711728207522No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG45420.11576504843678953No Hit
GTCTTAAGAGTTGCCAGCATACATTTTTGTTTGCATTTATTGATAAAGCA45380.11566309771161402No Hit
ATGTGATACCACCTTCCTCCTGCAAGAATAGCCGTAATCAAAAAAATCAG45040.11479651654762221No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT44320.11296140349446307No Hit
CTAATGATCAGGGAAATAGAAATCAAAACCATAATGTGATACCACCTTCC44300.1129104281318753No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA43720.11143214261683043No Hit
CACACATATATCACAGTTTCTTTATCCATTCATTCATTGATGGGCATTTG43700.11138116725424269No Hit
TTCTTATGCCTTTGTGTCTTCACAGCTTGGCTCCCACATATTAGTGAGGA43440.11071848754060187No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC43160.11000483246437333No Hit
TTCTTTATCCATTCATTCATTGATGGGCATTTGGGTTGGTTTCATGATTT42470.10824618245509579No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA42170.1074815520162795No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG42140.10740508897239785No Hit
CTTAAAATCTACTCTTTTAGCAAAAATCCTGAATACAAATAATTTTATTA40860.1041426657667816No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG40740.10383681359125507No Hit
ATATTAGTGAGGACATATGATGTTTTGTTTTCCATTCCTGAGTTATTTCA40710.10376035054737345No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT39870.10161938531868778No Hit
GTATTTGCTTACATGAGTAAATTCTTCAGTGGTGATTTGTGAGATTTTTG39810.1014664592309245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA68700.092.655034
TAACAAG74950.084.735175
CGGTGCA2900.082.498712
CAACGCA16900.072.498879
GTAGTAA92350.070.41851
ACAAGAG90300.070.090267
ACAACGC17600.070.027328
TAACACG554.0641917E-665.9080665
TGACGCC4550.062.14189145
GCACGCA954.5656634E-1061.0516742
AACAAGA104700.060.727326
TAGTAAC107550.059.5899542
AACACGA13100.059.216636
ACACGAG12950.058.2230267
CGACCAG805.878792E-754.3741535
AAACACG19400.053.8135875
GCCGACC2900.052.499176145
CAAGAGC93950.052.0108958
AGTAACA127300.050.572663
CGTGCGC1457.2759576E-1249.9992183
Produced by FastQC (version 0.11.5)