FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544792
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC99650.2811166353343158No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA71980.20305845871915756No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT63570.17933351237533823No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC45210.12753921809798713No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA39020.11007698053933772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC15100.0114.270411
GTAACAA89100.0110.66114
GCACGCA16900.0101.6706162
TAGTAAC101350.097.714882
TAACAAG101450.097.046875
CGCACTT20400.088.13645
AGTAACA115900.085.76063
CACGCAC20300.084.28493
AAACACG37600.083.297065
GAAACAC39450.079.023314
ACGCACT22750.078.7135244
ACAAGAG127550.077.4727867
CGGTGCA8800.074.147012
ACGTGCA19800.073.963922
CAAGAGC126500.067.742438
AACAAGA148750.066.3825456
GAAGCGG10600.065.659741
GTAGTAA156900.064.8750841
AACACGT16450.061.26086
TAGAAAC64500.060.809492
Produced by FastQC (version 0.11.5)