FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037876
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC54230.5225094327260674No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT53900.5193298621415275No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA40050.38588424821462297No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT27690.2667948772300352No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT23050.2220881877989278No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT19610.18894357322069302No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19550.1883654694780494No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT18000.17343112279308895No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA17920.17266031780289745No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT17920.17266031780289745No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT17600.16957709784213143No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG14880.14336972817562021No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA14070.1355653276499312No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC13920.13412006829332213No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT13800.13296386080803485No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT13470.1297842902234949No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA12830.12361785030196284No Hit
GGGTTTAGAACAGACTTAACCATTCCTCCATTACAGAGCCATGTCAACAC12720.12255799344044953No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC12610.12149813657893621No Hit
CCATTCCTCCATTACAGAGCCATGTCAACACCTGTGACAGTGTCCCACAG12560.12101638346006653No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG12420.1196674747272314No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT11170.1076236467554891No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA11010.10608203677510608No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT10790.10396232305207945No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC10780.10386597242830549No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT10590.10203531057660067No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC10470.10087910309131343No Hit
CAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAA10390.1001082981011219No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGCG100.007090901145.0145
ACGCCCC100.007090901145.0145
AGCACGA100.007090901145.02
AGTAGCC151.2300188E-4144.99998145
GTAACAA25650.0129.45424
CACGCAC3900.0117.115393
AGCACGC3900.0117.115391
TAACAAG28350.0116.869495
GCACGCA3950.0115.632912
GAAACAC7100.0114.366194
CAGGTCA203.866099E-4108.752
TTCGGAC203.866099E-4108.75145
AAACACG7700.0105.454555
GAGCAGA1400.0103.571431
ACGTGCA6800.0102.352942
TAGTAAC33400.099.85032
TAGAAAC9250.097.1891862
GGTGCAT1500.096.666673
TTCGGAA451.0917574E-896.666664145
AGTAACA36050.092.711523
Produced by FastQC (version 0.11.5)