FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400490
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA74130.21799799440668843No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT61840.1818561442615623No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG54270.15959464665386458No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA50170.14753756076330177No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG49960.14692000270549244No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT44170.12989304482589273No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC40130.11801240409470401No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38430.11301312457910478No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT38270.11254260415410719No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC38010.11177800846348615No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC37710.1108957826666157No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA37540.11039585471505577No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC37300.10969007407755942No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG36950.10866081064787722No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT34870.10254404512290875No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA34530.10154418921978892No Hit
GTATTGTCCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAA34290.10083840858229255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC10700.0113.153651
GCACGCA12000.0100.895332
GTAACAA80950.088.128054
TAGTAAC86450.083.192192
TAACAAG85850.082.844685
GTAGTAA88050.082.58621
CGGTGCA4000.079.749612
CACGCAC15600.078.541283
GCGGTGC12600.072.499651
AGTAACA100000.072.064653
TACGCGG1300.066.922745145
GCCGACC2200.065.90877145
GAAGCGG9000.063.638581
ACAACGC10900.060.5272338
AAACACG23450.059.0508845
ACAAGAG124050.057.625687
TAAGGCG3850.056.4932374
AACACGT9150.056.2565576
CGGCCTA3750.056.06649
CAAGAGG40300.054.6895568
Produced by FastQC (version 0.11.5)