FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071683
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC30260.2823596156699323No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC17590.16413435689471606No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA16060.14985774711365207No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT12370.11542592352402717No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT11390.10628142837014304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA256.4652104E-6115.99955145
GTAGTAA10600.057.4526061
AGCAACG9800.056.2242777
CAACGCA12750.054.0193989
TTATAGC10500.053.1664623
GCAACGC10250.053.0485768
TTTATAG10850.052.1196142
TAGCAAC11300.049.4024666
AGTCGTC450.0096394748.333157
TCTCGGG6250.047.55982145
TAGTAAC13100.045.9349372
GCCGACC1652.910383E-1143.939224145
TATAGCA13200.043.9392244
GCACGCA2150.043.837042
TATGGCG2400.042.2915085
ACAACGC3500.041.428418
TAGTAGT3900.040.8972783
CGGTGGG8300.039.307076145
ATAGCAA14400.039.2706835
TCGCCGG9350.038.769905145
Produced by FastQC (version 0.11.5)