FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219890
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA89950.4052002576704251No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC82850.3732166909171175No Hit
ATATATATGTATGTGTGTGTATTAGATGCAGGAGGCAGATAACGGAAGTT58270.26249048376270895No Hit
ACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAAAGTTCC41200.18559478172341873No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG31950.14392605038988418No Hit
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT31140.14027722094338008No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA31050.13987179544932407No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG29860.13451116947236125No Hit
CATATATATATATGCCCACATCCGTGCACAAACACACACATTCAACTGTA28910.13023167814621445No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT28150.126808085085297No Hit
ACAACAGCCAACACCACAGCCATCTCAATTGGTTAAATTTACACAATTTT28070.12644770686835835No Hit
ATATATATATGTATGTGTGTGTATTAGATGCAGGAGGCAGATAACGGAAG27870.12554676132601164No Hit
AGCACGCACTTGGACACATTCTGTATTCATCACTGCCAGATCAATATTGT27690.12473591033789963No Hit
GTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATA26860.12099698633716086No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG26550.11960052074652348No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT26520.11946537891517146No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA25210.11356418561280063No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC24580.11072620715440855No Hit
ATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATT24330.10960002522647518No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG22390.10086085346571226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA16700.086.391592
GTAGTAA110800.085.586011
AGCACGC17700.082.32991
TAGTAAC117850.080.6506652
GTAACAA121750.077.650354
AGTAACA122750.077.490273
CGCACTT17000.071.646535
TAACAAG131900.071.400165
ACGCACT21150.071.299714
AAGAGCG25250.068.9103859
ACAAGAG142550.065.099497
CAAGAGC141450.064.6319058
CTGTCGT18700.060.0931431
CACGCAC25000.058.5795753
CGGTGCA4350.058.332912
GTCGTCC19900.056.4694333
AGCGCGA400.006052028454.3746036
CGAGCGC400.006052028454.3746034
AACAAGA172950.053.950176
TGCGGCG553.2199748E-452.7268875
Produced by FastQC (version 0.11.5)