FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21356242
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC417330.19541359383359677No Hit
ACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAAAGTTCC372910.17461405428913945No Hit
GTAGTAACAAGAGCATAGGTCCTGAGGTAGCAGCTCCTGGTGGGGTTCAG295510.13837172289019764No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC273110.12788298615458657No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG259240.12138839782767025No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA255880.1198150873173286No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT251980.11798892333211058No Hit
GTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATA231080.1082025573600449No Hit
GTAGTAACAAGAGCAGGGCATGTATGAGTAGTTGAGAACGGTGAATAGGA221290.10361841750997203No Hit
ATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATT219720.10288326944412785No Hit
AGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACAACGCCCGGGTTA217660.10191868026219218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA622100.079.7130051
TAGTAAC631800.078.202292
CGTGCAT20450.060.977333
TAACAAG826900.058.8042345
CACGCAC79100.058.567633
GTAACAA839050.058.2983364
AAGAGCG206300.055.9824459
TCCGTGC22000.055.363298
AGCACGC86350.054.825691
AGTAACA930650.053.5729943
ACAAGAG921700.052.795597
GCGGTGC51450.049.178281
CAAGAGC918250.049.1725778
TCCGCGA450.00964221848.3328064
AACAAGA1013400.048.1182986
CAGCGGA146250.047.6390239
CCAGCGG146600.047.5252848
CGGTGCG11850.047.1091882
ACGTGCA69000.046.9668772
TCTCGGG42750.042.567085145
Produced by FastQC (version 0.11.5)