FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444657
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC73800.21424484353594567No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC63150.18332739660291286No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT56160.16303510044686598No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG55310.16056751078554413No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA53930.15656130639422156No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT52010.1509874568062945No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT50950.1479102273462931No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT50550.14674900868214164No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA47220.13708186330308067No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA45650.13252408004628616No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA44880.13028873411779462No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT42360.12297305653364037No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA42120.12227632533514947No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT42010.12195699020250783No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG40640.11797981627778904No Hit
GTAGTAACAAGAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAA40530.11766048114514739No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA39800.11554125708307098No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA38900.11292851508873017No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA38800.11263821042269231No Hit
AAAATGAACAATGCTACCTTCAACTATACAAACGTTAACCCTATTTCTCA37640.10927067629665303No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT36620.1063095687030668No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA35710.1036677962421222No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA35590.10331943064287678No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCGT259.3890243E-486.999273
CGTCAGA259.3890243E-486.999272
CGGTGCA4550.086.043232
GCCGACC1500.082.16598145
GGTGCAT5150.071.795513
GCACGCA8900.071.684792
CACGCAC9050.071.2977453
ACGTGCA14400.065.95432
AGCACGC10300.063.348981
GTAACAA136100.060.0876664
ACGATCC2200.059.317684145
TAACAAG139250.058.572225
TAGCCCG400.006052337654.3745465
GCGTCAG400.006052337654.3745461
TAGTAAC157050.052.6261062
TGACGCC2800.051.78528145
GTGCGGG1550.051.451184
AGTAACA164100.050.4535683
CGGTGCG8400.049.196022
CGGAGCA13750.049.035952
Produced by FastQC (version 0.11.5)