FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951776
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC105930.35886869464349597No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT90660.3071371269364613No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC67820.2297599817872359No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA65070.22044355669264878No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT61730.2091283349414048No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT53830.18236478648786358No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG46650.15804044751363247No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC44550.15092608653231138No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA41700.14127088234337565No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG41530.140694957882983No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGATCCCGGGCAAGATAAACGAGGGT37390.12666950337695002No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA36110.12233313096928763No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG35080.11884370629749683No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA33940.11498162462192252No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG32880.11139056622182712No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC31040.10515703088581248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC14850.0124.492811
GCACGCA15000.0122.281232
CACGCAC17400.0105.414853
CGGTGCA8050.097.265412
GCGGTGC13000.092.575331
TAACAAG82550.092.039615
AAACACG19650.091.1307455
GTAACAA84750.089.735924
GAAGCGG7300.088.38891
CGCACTT21550.087.46955
ACGCACT22400.085.121314
GAAACAC21250.083.927984
ACGTGCA11000.079.748632
TAGTAAC99400.077.312562
GTAGTAA103600.075.6478351
AACACGT6000.073.707076
ACAACGC6550.073.0521858
AGTAACA107250.071.3833853
TGACGCC2850.068.6842145
AACACGA20350.065.907966
Produced by FastQC (version 0.11.5)