FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639752
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT109170.6657714093350702No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT90050.5491684108328576No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT84790.5170903892783787No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT71970.43890783484331775No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT65650.4003654211124609No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC65560.39981655762578727No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT65150.3973161795198298No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA63060.3845703496626319No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT45320.27638325795608115No Hit
CACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA43500.26528401855890404No Hit
AGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA43390.2646131854085252No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG42400.25857568705511563No Hit
GCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAAC36460.2223506969346584No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT35550.21680107723606987No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT34660.21137342720118651No Hit
GCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAAC33750.20582380750259793No Hit
GTATAGTGCTTTGTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAA33300.20307949006922996No Hit
GAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC32990.20118896028179872No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT30690.18716244895569573No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT30580.1864916158053169No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT29880.18222267757563337No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA28440.17344086178885587No Hit
CATCTTGCTTTATCAAGGAAAACCAGTTAAAGTAGTGAAAAGGAAAAGGT27750.16923290839102498No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT26310.16045109260424747No Hit
AAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACA26090.15910942630348981No Hit
GGATGAAGTGGGCCATGTGTTCTAACTTTCCCTTGGCTCTACCCAGATCT25270.15410867009157483No Hit
ACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGCTGAAA24990.1524010947997014No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA24920.1519742009767331No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT24740.15087647400338589No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT24660.1503885953485649No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG24490.14935185320707034No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT24230.14776624757890217No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC23920.1458757177914709No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT23790.14508291497738682No Hit
GTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT23060.14063102225214544No Hit
GCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGTG22260.13575223570393571No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC21660.13209314579277842No Hit
AACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATT21600.13172723680166268No Hit
ATGTTATATTGGTCTACTGTTGTTTGCTTCAAAACTGTTTTGGCCTCATT21320.13001966150978927No Hit
CAACAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA21310.12995867667793667No Hit
GCTCTACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGC20530.12520185979343218No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT20090.12251852719191683No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA19860.12111587605930653No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC19850.1210548912274539No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA19800.12074996706819079No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT19610.11959125526299098No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC19500.11892042211261214No Hit
GTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT18420.11233406027252903No Hit
CACGTGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAATGA17740.10818709170655075No Hit
GCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTC17730.10812610687469812No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG17080.10416209280427773No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC16990.10361322931760414No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT16540.10086891188423616No Hit
AACACGTGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT16520.1007469422205309No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCC100.007091455144.998767
CGGGCCT100.007091455144.998768
GTAACAA49450.0128.871484
AGCACGC12750.0122.2538451
TAACAAG52450.0121.63865
CACGCAC13200.0119.1845863
GCACGCA13200.0118.6353452
GAGCAGA3450.0111.3758541
CGTCAGA404.818503E-9108.749072
GCGAGGC203.866635E-4108.749078
CGGTGCA2600.0108.749062
GAAACAC21350.0108.664164
AAACACG22000.0105.4536365
TAGTAAC61150.0104.806952
AGTAACA64000.0100.253043
ACGCACT15800.0100.0307854
TAGAAAC24950.099.959052
CGCACTT15950.099.090065
GTAGAAA25250.099.058551
ACAGCGT1100.092.2719354
Produced by FastQC (version 0.11.5)