FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487770
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA113660.32588158049412663No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA73120.20964685171327235No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT60670.1739506905558566No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG48860.14008951278323972No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG48410.138799290090803No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC39230.11247874716509403No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC36380.1043073367796615No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA35780.10258703985641254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA212900.057.6863171
TAGTAAC214200.057.166982
GTAACAA238250.051.152864
CTGTCGT11600.049.9996641
TGTCGTC11700.049.572322
AGTAACA246800.049.5276263
GTCGTCC11750.049.361373
TAACAAG249650.048.787985
ACAAGAG254600.046.3586857
CAAGAGC233050.046.0413138
AAGAGCG21900.045.3535779
AACAAGA269850.043.7656866
CAGGCGA13950.043.655629
ACAACGC31200.042.523758
CGTCCAG14650.039.5901765
AACAACG34200.039.217577
GCACGCA30900.039.1825872
CGTGCAC16450.038.7839363
GCTATCG1157.1481463E-637.8258328
TCTGTCG13900.037.5537038
Produced by FastQC (version 0.11.5)