FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3476451
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG323890.9316685320748084No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA161390.46423781034163863No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA160730.4623393224872147No Hit
GTAGTAACAAGAGCCTCTGGTTGAGGTCCTTAACTTCAGAATTTCCCTCT137380.39517312339509464No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG130250.3746637015738177No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG129560.3726789188169199No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT113560.3266549708308847No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA94300.27125364344269487No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT86160.24783895990479946No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT79220.2278760724658567No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA78820.22672547376620583No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG73390.2111060964184451No Hit
TTCTAATGCATAGCCAAAGTCAAGAATCACTAGAGCTCTCTTTAAAATTC72530.20863230921419573No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA70390.20247660617106353No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT66090.19010767014981658No Hit
AACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC64580.18576416005863453No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT64550.1856778651561607No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG64250.18481491613142254No Hit
CTTCATAATCAGAACAAACATGTGATTGAGTGGAACAAGCTAGTGCTAGC63170.18170829964236515No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC61440.1767319602663751No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG61250.17618542588404093No Hit
CATATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAG58930.16951195342606584No Hit
AAACAACAGCGATCCGAAATCCCCACTGCATAGACAGAAAGGCTGAGGCC58610.16859147446634512No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC54230.155992418705168No Hit
ACACAATACCTTCCCACAATAGCCACTTCATAATCAGAACAAACATGTGA52980.152396797768759No Hit
GTAGTAACAAGAGCAGGGGGAGGAGGTAACGATTGGTTCACTGCTTAATT52630.15139002390656447No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC51200.1472766335553126No Hit
CTCCTTGACACCTGACACAATACCTTCCCACAATAGCCACTTCATAATCA50410.1450042011235021No Hit
AGTTCACACACTGCCTGAACATTATAATCGCCTAGGGAACAACACACACA50200.14440013680618538No Hit
CACACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAATGCA50120.1441700170662552No Hit
TTACTATGCCTCATTGAGCCATGGTTTGCCCATCTTGAGGTGGACATGCC50110.14414125209876394No Hit
CACACACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAATG48060.13824443376305318No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA47980.138014314023123No Hit
GAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCC47260.1359432363637514No Hit
CACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAATGCATA46650.13418857334678383No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG45690.13142713646762172No Hit
CTTTAAAATTCATATGTGACATATCCTTTTAACCCCAAAACAACCACACT45370.13050665750790102No Hit
GTTCACACACTGCCTGAACATTATAATCGCCTAGGGAACAACACACACAC44980.1293848237757414No Hit
GAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGGCTGTGTGCATGAG44070.12676721173403566No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC43900.12627820728668404No Hit
CACACACACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAA43620.12547278819692842No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG42830.12320035576511792No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA42520.12230864177288851No Hit
TTCCTTATCTTTTCATAGCCCCTAGTTCACACACTGCCTGAACATTATAA41730.12003620934107802No Hit
CTTAAAATCTACTCTTTTAGCAAAAATCCTGAATACAAATAATTTTATTA40840.1174761272343548No Hit
ATATATTCCTATAAATTTAATTTTCTTGCTGACTTCATCCTTGGAGTTCT40790.11733230239689846No Hit
GAACAAGCTAGTGCTAGCCCTCCCTTTTCCACTAATTTGATGCATGATAT40430.11629676356721265No Hit
TTTTAAAGCTTTGTAGGTGATTCTAATGCATAGCCAAAGTCAAGAATCAC40230.11572146421738722No Hit
AGCTAGTGCTAGCCCTCCCTTTTCCACTAATTTGATGCATGATATTTGGG38700.1113204241912226No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC38620.11109030445129242No Hit
TCTTCACACAGCCCCCAACTCCAAACACGCCTCTCAGAAGGACTTTTGTA38180.10982464588167645No Hit
ACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAAAGTTCC37940.10913428666188593No Hit
CCTTGACACCTGACACAATACCTTCCCACAATAGCCACTTCATAATCAGA37930.10910552169439466No Hit
CACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAATGCATAGC37650.10830010260463904No Hit
ATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCTAG37360.10746591854739215No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT37050.10657420455516273No Hit
CACACACACACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCT37040.10654543958767144No Hit
GGAATATATAGTGTCTTTGAACACACAAAGTGGGGAATTATATTTCTAGC36740.10568249056293329No Hit
AACACGAGCCTCTGGTTGAGGTCCTTAACTTCAGAATTTCCCTCTAGATC35720.10274846387882355No Hit
GTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTA35390.10179921995161158No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG35270.10145404034171632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC159850.097.013852
GTAGTAA161250.096.756051
CGAGCCG3400.091.690675
GTAACAA184800.083.209764
TAACAAG184800.082.778215
AAGAGCG27400.074.880979
AGTAACA214350.072.381223
AACAAGA217200.070.530236
GCACGCA5350.070.4668962
AGCACGC5500.069.863251
CGAGCTA6350.069.645285
CAAGAGC211850.069.40258
AAGAGCC113200.068.464729
CGGTGCA3450.067.2462
ACAAGAG228100.067.128087
GAAGCGG10650.066.032491
GCGGTGC10150.064.285361
TAACACG2550.059.7055475
CGGCTAC502.0109946E-457.999686
GCCGACC1550.056.12872145
Produced by FastQC (version 0.11.5)