FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261220
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA96880.42844128390868647No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT83320.36847365581411806No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC79770.35277416615809165No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT68010.3007668426778465No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA59510.26317651533243114No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC38200.1689353534817488No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG35440.1567295530731198No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA34750.15367810297096257No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT31080.13744792634064795No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT28780.12727642600012382No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG27520.12170421277009756No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG26680.11798940395008005No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA26620.11772406046293593No Hit
CACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG26260.11613199954007129No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG24210.10706609706264758No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA23790.10520869265263884No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA23380.1033955121571541No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG22750.10060940554214098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC26150.0119.77031
GCACGCA26850.0116.107762
CACGCAC29250.0106.3331153
GTAACAA90150.0103.663114
TAACAAG93150.0100.7136845
CGCACTT31900.0100.227055
GAAACAC26050.097.965244
ACGCACT33250.096.593774
GTAGTAA98350.096.420741
TAGTAAC98100.095.705712
AAACACG27200.093.2902455
CGGTGCA6900.092.463582
AGTAACA106450.088.26663
GCGGTGC11750.085.148761
GTCTCGC7650.084.346221
CTCGCTC8250.079.090743
ACGTGCA16750.078.775952
CGACGAA2550.076.76454145
ACAAGAG124750.075.492827
AACAAGA130500.072.166516
Produced by FastQC (version 0.11.5)