FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741515
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT180570.658650417743474No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC132680.4839659823126993No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT100580.3668774382047882No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT80660.29421688373034616No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC79940.29159059862886033No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA66740.24344203843495293No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA62440.22775728018996796No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG50680.18486129019903227No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT50100.18274567164505756No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT46910.17110976959819663No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT44790.16337681902159937No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA40180.14656129913569688No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT38740.14130872893272514No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG37860.138098824919798No Hit
CACGTGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA36910.13463358763311528No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC35090.12799492251547046No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA33730.12303416176821941No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC32210.11748978210952704No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG32170.11734387738166671No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA31430.11464463991625068No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA31420.1146081637342856No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA31190.11376921154908873No Hit
GCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGTA29980.1093555935313139No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTATCCAAT29970.10931911734934881No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT29750.10851664134611703No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG29310.10691168933965345No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC28980.10570797533480576No Hit
CACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA28330.10333702350707548No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG28260.1030816902333199No Hit
ATATAAATCCTTACTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT28170.10275340459563417No Hit
AGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAACAA27550.10049188131379912No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG27490.10027302422200862No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC27470.10020007185807848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC30300.0126.335541
GCACGCA30400.0125.4429862
CACGCAC30900.0123.413173
CGGTGCA5350.0108.410272
GAAACAC20950.0106.240124
GTAACAA83550.0104.6489944
ACGCACT38450.0101.2544
CGCACTT38200.0101.15755
AAACACG22050.0100.940175
TAACAAG89900.097.257225
TAGTAAC102350.085.993392
GTAGTAA103800.085.7699661
GCGGTGC17650.084.616831
AGTAACA106350.082.486353
GGTGCGT6300.080.5548553
ACGTGCA16100.080.154592
GCCGACC1000.079.74931145
CGGTGCG6400.079.296192
ACAAGAG112250.078.409137
TGACGCC1250.075.399345145
Produced by FastQC (version 0.11.5)