FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641396
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC142350.5389195713175912No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC74300.2812906508528066No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT68910.26088477456617637No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT46170.1747939347223968No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT44640.16900154312340898No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA44390.1680550739078881No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG41940.15877967559578343No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC40270.15245726123610392No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT36570.13844951684639487No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA36070.1365565784153531No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA35240.13341430061982376No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA31760.12023944913977305No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG30230.11444705754078525No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA29720.11251626034112265No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA28910.10944970008283499No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT28500.10789749056938074No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA28450.10770819672627657No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT27850.10543667060902644No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT27560.10433876631902221No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT27560.10433876631902221No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT27100.10259726296246378No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA26800.10146149990383872No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA100.0070916433144.99913145
GTAACAA60850.0115.689524
CGGTGCA5850.0109.059172
GGTGCAT5300.0105.329563
GCACGCA4700.0103.350452
TAACAAG68500.0102.663615
AGCACGC4750.0102.262541
GTCTCGC1550.098.225211
CACGCAC4950.098.130733
CTCGCTC1600.095.155683
CCGAATT406.120481E-790.624464
TAGTAAC91150.077.550282
AAACACG20250.074.468695
ACGTGCA9600.074.009972
GAAACAC20750.072.674264
ACACTCG502.3073735E-672.4995654
CTGTCGT1850.070.5401159
AGTAACA100800.070.4137653
CGTGCAG651.399003E-766.9226763
ACAAGAG107250.065.5031057
Produced by FastQC (version 0.11.5)