FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760765
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC22210.29194297844932404No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT20000.2628932719039388No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT18090.2377869644371126No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16670.219121542131933No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG15740.20689700498839983No Hit
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA13180.17324666618469567No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT12640.16614854784328933No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA12620.16588565457138538No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC10030.1318409758598253No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT9720.12776613014531427No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG9510.1250057507903229No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA9030.11869631226462836No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG8440.11094096074346217No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG8290.10896926120418263No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA7870.10344850249419991No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG7760.10200258949872826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGA100.007091165144.995676
ACGAGCG203.8651467E-4108.753899
CATAGCG203.8661575E-4108.746757
CGGTGCA1900.0106.838912
GTCTCGC900.096.663781
GTAACAA24900.096.081464
AGCACGC3800.095.3918841
GCACGCA3900.092.945942
TAACAAG26150.092.597615
CACGCAC4000.092.434733
AAACACG6450.092.1677865
TGACGCC800.090.62824145
GAAACAC7000.083.890354
TAGTAAC29550.081.452722
ACGTGCA2500.081.197572
GTAGTAA30650.079.948671
AGTAACA31550.076.5191043
TAGCGAG300.00193528972.5025949
TTGCGCC406.660981E-572.502594145
CATCCGA502.3061184E-672.497832
Produced by FastQC (version 0.11.5)