FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4202061
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT74510.17731774955194607No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA69470.16532363523518578No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT62410.14852235605337477No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA60720.1445005201019214No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT54270.12915090951797226No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT52800.12565262617558384No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT48240.11480080846041979No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG43330.10311606613992515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGCC3050.030.901606145
CAACGCA86350.030.8978449
ACGATCC10450.030.526283145
ACGTGCA14250.028.490862
GAGCATC10350.028.0189594
TCGATAC1050.007711253627.619019145
ATTGTAC10350.026.6180113
AACAACG105400.026.3445367
ACAACGC105050.026.1562548
TGCACCG15300.023.6925055
GCGTCGG1851.8320383E-423.51349145
CGTGCAC11150.023.4077683
TAACAAG346500.022.8481885
GTAACAA349300.022.7273034
TAGTAAC356300.022.504622
GTGCACC12050.022.2611224
TCGAGGG1650.002602249621.969673145
AGTAACA367550.021.7171733
TCGCATG3457.517883E-821.0147199
TATGTGG61450.020.764586
Produced by FastQC (version 0.11.5)